Wisnu Tafroji, Indonesia
Eijkman Institute for Molecular Biology Molecular BacteriologyPresenter of 1 Presentation
ANTIMICROBIAL ACTIVITY OF MEDICINAL PLANT EXTRACTS IN INDONESIA AGAINST MULTI-DRUG RESISTANT STRAIN OF STREPTOCOCCUS PNEUMONIAE (ID 513)
Author Of 5 Presentations
MULTILOCUS SEQUENCE TYPE ANALYSIS OF SEROTYPE 19F OF STREPTOCOCCUS PNEUMONIAE STRAINS PRIOR PNEUMOCOCCAL VACCINATION INTRODUCTION IN INDONESIA (ID 515)
Abstract
Background
Streptococcus pneumoniae serotype 19F is one of the invasive serotypes causing IPD in Indonesia where it is commonly found as multi-drug resistant (MDR). However, relationship between its genetic variation and multi-drug resistant is still not well described. Therefore, we performed multilocus sequence typing on multidrug resistant serotype 19F to describe sequence type of multi-drug resistance serotype 19F in Indonesia.
Methods
We used 25 archived isolates previously described as serotype 19F. Antimicrobial susceptibility testing was performed using disk diffusion according to CLSI guidelines. Multi-locus sequence typing was performed by sequencing targeting seven house-keeping genes; aroE, gdh, gki, recP, spi, xpt, and ddl. Sequence type (ST) was determined by allele number combination of seven house-keeping genes.
Results
S. pneumoniae serotype 19F isolates were resistant to tetracycline (89%), Sulfametoxazole/Trimethophrim (72%), macrolide (52%), and chloramphenicol (60%) where 56% of isolates was MDR. We identified 5 sequence types where ST 9192 as dominant sequence type (44%) while others were ST 320 (20%), ST 242 (16%), ST 236 (12%), and ST 271 (4%) belonged to MDR strain.
Conclusions
Serotype 19F has been resistant to common antibiotic used in Indonesia where sequence type 320, 242, 236 and 271 are sequence type for MDR strain of serotype 19F
COMPARISONS OF STREPTOCOCCUS PNEUMONIA SEROTYPE ON NASOPHARYNGEAL CARRIAGE BETWEEN UNDER-FIVE CHILDREN WITH COMMUNITY ACQUIRED PNEUMONIA AND HEALTHY (ID 1196)
Abstract
Background
Streptococcus pneumonia colonization in nasopharyng consist of many serotypes, strongly related to high morbidity and mortality of pneumonia in children under-five.This study objective is to compare between S. pneumonia serotypes on nasopharyngeal carriage among children with pneumonia infection and healthy.
Methods
We collected the nasopharyngeal (NP) swab among 130 under-five years of age pneumonia children at M. Djamil hospital and healthy children as control from two daycares in Padang, respectively. Demographic and clinical data were recorded during NP swab collection.The isolate of Streptococcus pneumoniae has been performed by culture on 8% sheep-blood agar plate, were identified serotype by PCR methods.
Results
Overall we found that 19F (21%) and untypeable Streptococcus pneumonia serotype (21%) are the most frequent from PCR identification, followed by 6C, 14 and 34 serotype. Comparing pneumonia cases and healthy children, 19F serotype (22%) more frequent among control healthy children than pneumonia cases (17%). We found untypeable and 6B serotype are the most frequent among pneumonia patiens, but not in control group.
Conclusions
We found the same proportion of 19F serotype Streptococcus pneumoniae among pneumonia patiens and healthy, but very high untypeable serotype in pneumonia patiens.