Miftahuddin M. Khoeri, Indonesia
Eijkman Institute for Molecular Biology Molecular BacteriologyPresenter of 1 Presentation
MULTILOCUS SEQUENCE TYPE ANALYSIS OF SEROTYPE 19F OF STREPTOCOCCUS PNEUMONIAE STRAINS PRIOR PNEUMOCOCCAL VACCINATION INTRODUCTION IN INDONESIA (ID 515)
Abstract
Background
Streptococcus pneumoniae serotype 19F is one of the invasive serotypes causing IPD in Indonesia where it is commonly found as multi-drug resistant (MDR). However, relationship between its genetic variation and multi-drug resistant is still not well described. Therefore, we performed multilocus sequence typing on multidrug resistant serotype 19F to describe sequence type of multi-drug resistance serotype 19F in Indonesia.
Methods
We used 25 archived isolates previously described as serotype 19F. Antimicrobial susceptibility testing was performed using disk diffusion according to CLSI guidelines. Multi-locus sequence typing was performed by sequencing targeting seven house-keeping genes; aroE, gdh, gki, recP, spi, xpt, and ddl. Sequence type (ST) was determined by allele number combination of seven house-keeping genes.
Results
S. pneumoniae serotype 19F isolates were resistant to tetracycline (89%), Sulfametoxazole/Trimethophrim (72%), macrolide (52%), and chloramphenicol (60%) where 56% of isolates was MDR. We identified 5 sequence types where ST 9192 as dominant sequence type (44%) while others were ST 320 (20%), ST 242 (16%), ST 236 (12%), and ST 271 (4%) belonged to MDR strain.
Conclusions
Serotype 19F has been resistant to common antibiotic used in Indonesia where sequence type 320, 242, 236 and 271 are sequence type for MDR strain of serotype 19F
Author Of 4 Presentations
PROPORTION OF PNEUMOCOCCAL PNEUMONIA AND SEROTYPE DISTRIBUTION IN COMMUNITY-ACQUIRED PNEUMONIA PATIENTS WITH UNDERLYING COMORBIDITIES IN JAKARTA, INDONESIA (ID 269)
ANTIMICROBIAL ACTIVITY OF MEDICINAL PLANT EXTRACTS IN INDONESIA AGAINST MULTI-DRUG RESISTANT STRAIN OF STREPTOCOCCUS PNEUMONIAE (ID 513)
SEROTYPE DISTRIBUTION AND ANTIMICROBIAL SUSCEPTIBILITY OF STREPTOCOCCUS PNEUMONIAE CARRIED BY HEALTHY BAJAU CHILDREN UNDER 5 YEARS OF AGE IN KOTABARU, SOUTH KALIMANTAN (ID 395)
Abstract
Background
The indigenous Bajau people (Sea Nomads) have lived an entirely marine-dependent existence and renowned for their extraordinary breath-holding abilities. Kotabaru in South Kalimantan is one of the island inhabited by Bajau people. We investigated nasopharyngeal carriage of S. pneumoniae in healthy Bajau children <5 in Kotabaru, South Kalimantan, Indonesia.
Methods
Serotyping was performed using Conventional multiplex PCR. Antimicrobial susceptbility test using disk diffusion and microdulution were employed to all pneumococcal and MDR isolates, respectively.
Results
We found 45% (180/399) of S. pneumoniae. Cigarette exposure contributed to colonization of S. pneumoniae (OR = 1,87). The most common serotypes were 6A/6B (18%), 15B/15C (17%) and 19F (16%). We identified 46% (86/185) was vaccine-type and 40% as non-vaccine type (74/185). The majority of 18% (34/185) defined as Multidrug Resistant pneumococcus were 19F serotype (74%), followed by 6A/6B (9%), 19A (9%), 14 (6%), and 23F (3%). Tetracycline (89%), trimethoprim/sulfamethoxazole (79%), cefuroxime (76%), azithromycin (76%) and erythromycin (76%) showed high prevalence of resistance among MDR isolates with MICs90 were 8, 4/76, >4, and >2 μg/mL, respectively.
Conclusions
Almost half of the identified serotype were vaccine type. All of the multidrug resistant isolates were vaccine type and dominated by 19F. Serotype 15B/15C was the second most common serotype.
MULTILOCUS SEQUENCE TYPE ANALYSIS OF SEROTYPE 19F OF STREPTOCOCCUS PNEUMONIAE STRAINS PRIOR PNEUMOCOCCAL VACCINATION INTRODUCTION IN INDONESIA (ID 515)
Abstract
Background
Streptococcus pneumoniae serotype 19F is one of the invasive serotypes causing IPD in Indonesia where it is commonly found as multi-drug resistant (MDR). However, relationship between its genetic variation and multi-drug resistant is still not well described. Therefore, we performed multilocus sequence typing on multidrug resistant serotype 19F to describe sequence type of multi-drug resistance serotype 19F in Indonesia.
Methods
We used 25 archived isolates previously described as serotype 19F. Antimicrobial susceptibility testing was performed using disk diffusion according to CLSI guidelines. Multi-locus sequence typing was performed by sequencing targeting seven house-keeping genes; aroE, gdh, gki, recP, spi, xpt, and ddl. Sequence type (ST) was determined by allele number combination of seven house-keeping genes.
Results
S. pneumoniae serotype 19F isolates were resistant to tetracycline (89%), Sulfametoxazole/Trimethophrim (72%), macrolide (52%), and chloramphenicol (60%) where 56% of isolates was MDR. We identified 5 sequence types where ST 9192 as dominant sequence type (44%) while others were ST 320 (20%), ST 242 (16%), ST 236 (12%), and ST 271 (4%) belonged to MDR strain.
Conclusions
Serotype 19F has been resistant to common antibiotic used in Indonesia where sequence type 320, 242, 236 and 271 are sequence type for MDR strain of serotype 19F