Jeny Lim, Indonesia
Eijkman Institute for Molecular Biology Molecular BacteriologyAuthor Of 1 Presentation
MULTILOCUS SEQUENCE TYPE ANALYSIS OF SEROTYPE 19F OF STREPTOCOCCUS PNEUMONIAE STRAINS PRIOR PNEUMOCOCCAL VACCINATION INTRODUCTION IN INDONESIA (ID 515)
Abstract
Background
Streptococcus pneumoniae serotype 19F is one of the invasive serotypes causing IPD in Indonesia where it is commonly found as multi-drug resistant (MDR). However, relationship between its genetic variation and multi-drug resistant is still not well described. Therefore, we performed multilocus sequence typing on multidrug resistant serotype 19F to describe sequence type of multi-drug resistance serotype 19F in Indonesia.
Methods
We used 25 archived isolates previously described as serotype 19F. Antimicrobial susceptibility testing was performed using disk diffusion according to CLSI guidelines. Multi-locus sequence typing was performed by sequencing targeting seven house-keeping genes; aroE, gdh, gki, recP, spi, xpt, and ddl. Sequence type (ST) was determined by allele number combination of seven house-keeping genes.
Results
S. pneumoniae serotype 19F isolates were resistant to tetracycline (89%), Sulfametoxazole/Trimethophrim (72%), macrolide (52%), and chloramphenicol (60%) where 56% of isolates was MDR. We identified 5 sequence types where ST 9192 as dominant sequence type (44%) while others were ST 320 (20%), ST 242 (16%), ST 236 (12%), and ST 271 (4%) belonged to MDR strain.
Conclusions
Serotype 19F has been resistant to common antibiotic used in Indonesia where sequence type 320, 242, 236 and 271 are sequence type for MDR strain of serotype 19F