Sonia Paytubi (Spain)

Institut Catala d'Oncologia Cancer Epidemiology Research Program-INCALAB

Presenter of 2 Presentations

 Conference Calendar
Clinical Research / HPV Diagnostics and Biomarkers for Early Detection and Prognosis of HPV-related Cancers ePoster

COMPARISON OF HPV GENOTYPE SPECIFIC DNA AND E6/E7 MRNA DETECTION AND PREVALENCE OF ANAL HPV INFECTION IN HIV-INFECTED MEN WHO HAVE SEX WITH MEN (ID 1301)

Session Date
07/21/2020
Session Time
10:00 - 17:00
Room
ePoster
Session Type
Poster Viewing - 20-24 July
Session Name
Clinical Research / HPV Diagnostics and Biomarkers for Early Detection and Prognosis of HPV-related Cancers
Lecture Time
10:32 - 10:33
Presenter
Authors

Abstract

Introduction

Human Papiloma Virus (HPV) infection and the development of anal cancer (AC) is increasing in HIV-positive men who have sex with men (MSM). Most subjects who screen positive by HPV-DNA testing or cytology do not have concurrent precancer. The challenge is differentiating the screen-positive subjects with benign, transient HPV infections from those that have precancerous lesions with a high risk to progress to cancer. We aim to estimate the anal HPV prevalence and the performance of three HPV detection tests.

Methods

The ELAVI67 project, a prospective longitudinal study, includes samples from 347 HIV-MSM recruited at baseline in Barcelona. Baseline samples have already been collected and follow-up samples are currently being collected every 6/12 months after the baseline during minimum 24 months. The ELAVI67 cohort underwent anal smear and HRA with biopsy of suspected dysplasia areas. Baseline anal smear samples were tested by anal liquid-based cytology, HPV DNA detection performed by both Linear Array (LA) (37 HPV genotypes) and Hybrid Capture®2 (HC2) (13 high-risk (HR-HPV) genotypes), and E6/E7-mRNA test using Aptima® (14 HR-HPV genotypes).

Results

Prevalence HR-HPV was 40.9% by HC2, 79.3% by LA and 50.1% by Aptima. The overall agreement and ki between LA and HC2 for the common genotypes was poor (66%, 0.325). Whereas, the concordance and ki for shared genotypes between LA and Aptima was higher (74%,0.475) and increasingly better as the genotypes compared were restricted to HPV16/18/45 or to HPV16. For the identification of high-grade lesions (HSIL), the detection of HR-HPV by LA showed the most sensitive values (95%), although the specificity dropped to a 28% (AUC,0.61). The test that displayed a better performance was Aptima, showing a sensitivity of 80% and a specificity of 63% (AUC,0.71).

Conclusions

Preliminary results indicate that E6/7-mRNAtest perform better than HC2 and LA and could be considered for the detection of HSIL.

Hide
Clinical Research / Diagnosis and Management of Oropharyngeal, Head and Neck Cancer ePoster

ASSESSMENT OF ISOTHERMAL AMPLIFICATION AMPFIRE ASSAY FOR DETECTION AND GENOTYPING OF HPV IN FORMALIN-FIXED PARAFFIN-EMBEDDED HEAD AND NECK CANCER SAMPLES (ID 165)

Session Date
07/21/2020
Session Time
10:00 - 17:00
Room
ePoster
Session Type
Poster Viewing - 20-24 July
Session Name
Clinical Research / Diagnosis and Management of Oropharyngeal, Head and Neck Cancer
Lecture Time
10:02 - 10:03
Presenter
Authors

Abstract

Introduction

Infection with high risk HPV is etiologically linked to a group of oropharyngeal squamous cell carcinomas (OPSCCs) that show better clinical outcome and response to treatment. The combination of p16INK4A overexpression and HPV-DNA testing increases diagnostic accuracy and have a better prognostic value. The aim of this study was to assess the AmpFire HPV Multiplex and Genotyping Tests, for formalin-fixed paraffin-embedded (FFPE) samples.

Methods

Extracted DNA of the entire collection of OPSCC FFPE specimens collected between 2014-2019 at Hospital de Bellvitge summing a total of 160, plus 23 samples of other head and neck (HN) localizations were routinely tested using the Linear Array HPV Genotyping Test (LA). Additionally, viral DNA was detected and 15 HR- HPV types genotyped using the AmpFire HPV Multiplex and Genotyping Tests (Atila Biosystems). The DNA was amplified and real-time fluorescent detected using a 60ºC isothermal reaction for 74 min.

Results

LA and AmpFire HPV Multiplex tests showed, for total samples and OPSCC, a positive agreement of 98.89% and a 99.36% and a kappa index of 0.972 and 0.984, respectively. An overall concordance of 100% for the presence of HPV16 and 18 was observed. Amongst the samples where the test detected “Other HR-HPV genotypes”, the AmpFire Genotyping test was performed. The main disagreement was found for genotypes HPV45 and HPV52. It is worth mentioning that LA is unable to identify HPV52 alone in samples containing HPV33, HPV35, and/or HPV58 as a cross-reactive probe for all these genotypes is used. Moreover, 145 OPSCC samples had the p16INK4A IHC test done. The agreement observed between positives and negatives for HPV-DNA and p16 INK4A was 93.8% and a ki of 0.848.

Conclusions

The AmpFire HPV Tests are simple sample-to-answer and low cost assays for detection and genotyping of HPV in HN FFPE samples.

Hide