Poster Viewing - 20-24 July

ASSESSMENT OF ISOTHERMAL AMPLIFICATION AMPFIRE ASSAY FOR DETECTION AND GENOTYPING OF HPV IN FORMALIN-FIXED PARAFFIN-EMBEDDED HEAD AND NECK CANCER SAMPLES (ID 165)

Session Name
Clinical Research / Diagnosis and Management of Oropharyngeal, Head and Neck Cancer
Presenter

Abstract

Introduction

Infection with high risk HPV is etiologically linked to a group of oropharyngeal squamous cell carcinomas (OPSCCs) that show better clinical outcome and response to treatment. The combination of p16INK4A overexpression and HPV-DNA testing increases diagnostic accuracy and have a better prognostic value. The aim of this study was to assess the AmpFire HPV Multiplex and Genotyping Tests, for formalin-fixed paraffin-embedded (FFPE) samples.

Methods

Extracted DNA of the entire collection of OPSCC FFPE specimens collected between 2014-2019 at Hospital de Bellvitge summing a total of 160, plus 23 samples of other head and neck (HN) localizations were routinely tested using the Linear Array HPV Genotyping Test (LA). Additionally, viral DNA was detected and 15 HR- HPV types genotyped using the AmpFire HPV Multiplex and Genotyping Tests (Atila Biosystems). The DNA was amplified and real-time fluorescent detected using a 60ºC isothermal reaction for 74 min.

Results

LA and AmpFire HPV Multiplex tests showed, for total samples and OPSCC, a positive agreement of 98.89% and a 99.36% and a kappa index of 0.972 and 0.984, respectively. An overall concordance of 100% for the presence of HPV16 and 18 was observed. Amongst the samples where the test detected “Other HR-HPV genotypes”, the AmpFire Genotyping test was performed. The main disagreement was found for genotypes HPV45 and HPV52. It is worth mentioning that LA is unable to identify HPV52 alone in samples containing HPV33, HPV35, and/or HPV58 as a cross-reactive probe for all these genotypes is used. Moreover, 145 OPSCC samples had the p16INK4A IHC test done. The agreement observed between positives and negatives for HPV-DNA and p16 INK4A was 93.8% and a ki of 0.848.

Conclusions

The AmpFire HPV Tests are simple sample-to-answer and low cost assays for detection and genotyping of HPV in HN FFPE samples.

Hide

Previous ePoster
PERCEPTION OF SINGAPORE WOMEN ON CERVICAL CANCER, CERVICAL CANCER SCREENING AND HPV PRIMARY SCREENING (ID 150)

Next ePosters
POPULATION-BASED HUMAN PAPILLOMAVIRUS INFECTION AND GENOTYPE DISTRIBUTION AMONG WOMEN IN RURAL AREAS OF SOUTH CENTRAL ETHIOPIA (ID 166)
ACCEPTABILITY AND UPTAKE OF HPV SELF-SAMPLING AS AN ALTERNATIVE METHOD FOR CERVICAL CANCER SCREENING IN TORONTO, CANADA (ID 170)
DETECTION OF HIGH RISK HUMAN PAPILLOMAVIRUS (HR-HPV) IN PRECANCEROUS LESIONS OF CERVICAL CANCER (ID 176)
CHADOX1 AND MVA HETEROLOGOUS PRIME BOOST VACCINATION IN LOW-GRADE HPV-RELATED CERVICAL LESIONS (ID 182)
PRACTICE-, PROVIDER-, AND PATIENT-LEVEL FACILITATORS OF AND BARRIERS TO HPV VACCINE PROMOTION AND UPTAKE IN THE STATE OF GEORGIA (ID 184)
TRIAGE OF PRIMARY HPV TESTING COMBINED WITH SELF-SAMPLING USING HPV 16/18 GENOTYPING AND/OR CYTOLOGY. A SUB-ANALYSIS OF THE GRECOSELF STUDY (ID 192)
‘SCREEN AND TREAT’ PROGRAMME FOR CERVICAL CANCER PREVENTION IN PUNE, INDIA (ID 193)
PRIMARY CERVICAL CANCER SCREENING USING SELF-SAMPLING FOR HPV DNA TESTING: EVIDENCE OF ACCEPTANCE FROM A NATIONWIDE CROSS-SECTIONAL STUDY IN GREECE (ID 194)
ARTIFICIAL INTELLIGENCE AND ORAL BRUSH SAMPLING FOR MICROBIOLOGIC DIAGNOSIS IN GENERAL DENTAL PRACTICE, A NEW ENTITY TOWARDS EFFICIENT EXPLAINABLE CYTOLOGY. (ID 198)
HPV GENOTYPING OF ANAL SPECIMENS FROM HIV+MSM PARTICIPANTS: VALIDATING AMPFIRE HPV GENOTYPING ASSAY (ATILA BIOSYSTEM) AGAINST LINEAR ARRAY HPV GENOTYPING TEST (ROCHE). (ID 205)