Presenter of 1 Presentation
IS HUMAN PARVOVIRUS 4 EMERGING AS AN ETIOLOGICAL AGENT OF ACUTE ENCEPHALITIS SYNDROME IN INDIA?
Abstract
Background
Acute encephalitis syndrome (AES) continues to be a ‘major health threat’ in India. Japanese encephalitis (JE) virus is the most commonly identified virus, but in majority of cases the etiological virus remains the mystery. Parvovirus 4 (Parv4) is a new virus but its association with any disease and its spread in the human population has not been clearly assessed.
Aims
To find any association of Parv4 in causing acute encephalitis.
Methods
CSF and serum samples of patients presenting as Acute encephalitis syndrome are routinely tested for the presence of possible etiological as part of routine viral diagnostic workup in Virology laboratory, King George’s Medical University, Lucknow, India. The cohorts of CSF samples positive for any of tested viruses (cohort 1;n=12) and CSF samples negative for tested virus (cohort 2;n=9) were further subjected to Parv4 detection by real time PCR method. PARV4 positive Samples were sequenced and phylogenetically analayzed.
Results
In cohort 1, 8/12 (66.7%) and in cohort 2, 4/9 (44.4%) were detected positive for Parv4. Two of these parv4 positive samples (one from each cohort) were sequenced by next generation sequencing. The sequences are available in GenBank under accession no. KM390024 and KM390025.
Conclusions
Present study discusses its detection in patients of acute encephalitis syndrome; however, its association as causative agent is yet to be elucidated. Whether it is just a co infecting virus (suggestive as in patient 1) or a AES causing virus (suggestive as in patient 2), is an important research question.