KING GEORGES MEDICAL UNIVERSITY, LUCKNOW, INDIA
Microbiology
I began my career as a Research scholar when I joined King George's Medical University, Lucknow. My PhD work is broadly focused on Hepatitis C virus in patient undergoing hemodialysis under eminent guidance of Prof. S. N. Sankhwar (HOD, Urology, KGMU). I have completed more than 12 years of academic progress post master degree (M.Sc. in Biotechnology). My specific research areas are medical microbiology, molecular biology, human genetics and DNA mapping, diagnostic assay development, Acute Encephalitis Syndrome, Viral Hepatitis and microbial pathogen Microbiome. I have also worked on genomes of more than 45 pathogens. During this period, I have designed and developed molecular diagnostics assays for diagnosis of different viruses e.g HSV, HBV, HCV, DENV. I have also done a large no. of partial/whole gene sequencing using Sanger sequencing method and NGS technology. I have submitted many whole genome sequences of different viruses and more than 1000 sequences of various pathogens in NCBI GeneBank and more than 450 Covid-19 whole sequences. I am also involved with studies on human immune response to viral invasion. I am academically inclined research scientist with 53 article publications and four patents of different novel diagnostic kits for HBV, HCV, HSV-1, HSV-2, human parvovirus 4 and human parvovirus B19. Received many awards and honors. I have been involved in teaching and training of faculties, scientists, MD’s doctors, PhD’s and laboratory technicians. I had an opportunity of conducting an international workshop on virology in Singapore in the year 2017 and a similar workshop on NGS in national Microcon- 2019. Delivered a talk on CRISPR in microcon-2018. Trained participants from DHR labs across India for molecular and serological testing of scrub typhus. I have been involved training all the laboratories for covid-19 testing for the state of Uttar Pradesh. Appointed by state nodal officer as an Incharge of covid-19 genomic surveillance in U.P. I am a good team player with experience of working in a team consisting of more than 40-50 people. As a scientist, I have an experience of leading a team of more than 50 persons. I have been actively associated with many MD, DM, PhD thesis and intra and extramural/multicentric projects. Vision and contribution: Development of low cost molecular diagnostic assays affordable for poor section of the society. I want to develop a Point-of-Care testing's for diagnosis of different diseases/syndrome. I have a keen interest in infectious diseases birth defects screening which included neonatal screening and I want to work on source tracking of the infectious diseases. Development of therapy especially cancer therapies via oncolytic viruses, stem cell therapy, drug delivery via nanotechnology. I have a strong belief in viral flora of human body which may be in symbiotic relationship and may have beneficial effect on humans.

Presenter of 1 Presentation

IS HUMAN PARVOVIRUS 4 EMERGING AS AN ETIOLOGICAL AGENT OF ACUTE ENCEPHALITIS SYNDROME IN INDIA?

Session Type
Oral Presentations
Date
Thu, 24.02.2022
Session Time
10:00 AM - 11:00 AM
Room
Sala D
Session Icon
Pre-Recorded with Live Q&A
Lecture Time
10:20 AM - 10:30 AM

Abstract

Background

Acute encephalitis syndrome (AES) continues to be a ‘major health threat’ in India. Japanese encephalitis (JE) virus is the most commonly identified virus, but in majority of cases the etiological virus remains the mystery. Parvovirus 4 (Parv4) is a new virus but its association with any disease and its spread in the human population has not been clearly assessed.

Aims

To find any association of Parv4 in causing acute encephalitis.

Methods

CSF and serum samples of patients presenting as Acute encephalitis syndrome are routinely tested for the presence of possible etiological as part of routine viral diagnostic workup in Virology laboratory, King George’s Medical University, Lucknow, India. The cohorts of CSF samples positive for any of tested viruses (cohort 1;n=12) and CSF samples negative for tested virus (cohort 2;n=9) were further subjected to Parv4 detection by real time PCR method. PARV4 positive Samples were sequenced and phylogenetically analayzed.

Results

In cohort 1, 8/12 (66.7%) and in cohort 2, 4/9 (44.4%) were detected positive for Parv4. Two of these parv4 positive samples (one from each cohort) were sequenced by next generation sequencing. The sequences are available in GenBank under accession no. KM390024 and KM390025.

Conclusions

Present study discusses its detection in patients of acute encephalitis syndrome; however, its association as causative agent is yet to be elucidated. Whether it is just a co infecting virus (suggestive as in patient 1) or a AES causing virus (suggestive as in patient 2), is an important research question.

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