Korrie Salsabila, Indonesia
Eijkman Institute for Molecular Biology Molecular BacteriologyAuthor Of 2 Presentations
SEROTYPE DISTRIBUTION AND ANTIMICROBIAL SUSCEPTIBILITY OF STREPTOCOCCUS PNEUMONIAE CARRIED BY HEALTHY BAJAU CHILDREN UNDER 5 YEARS OF AGE IN KOTABARU, SOUTH KALIMANTAN (ID 395)
Abstract
Background
The indigenous Bajau people (Sea Nomads) have lived an entirely marine-dependent existence and renowned for their extraordinary breath-holding abilities. Kotabaru in South Kalimantan is one of the island inhabited by Bajau people. We investigated nasopharyngeal carriage of S. pneumoniae in healthy Bajau children <5 in Kotabaru, South Kalimantan, Indonesia.
Methods
Serotyping was performed using Conventional multiplex PCR. Antimicrobial susceptbility test using disk diffusion and microdulution were employed to all pneumococcal and MDR isolates, respectively.
Results
We found 45% (180/399) of S. pneumoniae. Cigarette exposure contributed to colonization of S. pneumoniae (OR = 1,87). The most common serotypes were 6A/6B (18%), 15B/15C (17%) and 19F (16%). We identified 46% (86/185) was vaccine-type and 40% as non-vaccine type (74/185). The majority of 18% (34/185) defined as Multidrug Resistant pneumococcus were 19F serotype (74%), followed by 6A/6B (9%), 19A (9%), 14 (6%), and 23F (3%). Tetracycline (89%), trimethoprim/sulfamethoxazole (79%), cefuroxime (76%), azithromycin (76%) and erythromycin (76%) showed high prevalence of resistance among MDR isolates with MICs90 were 8, 4/76, >4, and >2 μg/mL, respectively.
Conclusions
Almost half of the identified serotype were vaccine type. All of the multidrug resistant isolates were vaccine type and dominated by 19F. Serotype 15B/15C was the second most common serotype.
DIFFERENCES IN SEROTYPE DISTRIBUTION AND RISK FACTORS OF STREPTOCOCCUS PNEUMONIAE COLONIZATION IN CHILDREN UNDER FIVE YEARS OF AGE BETWEEN TWO SEA NOMADS POPULATION IN INDONESIA (ID 1060)
Abstract
Background
S. pneumoniae isolation from indigenous population prior to pneumococcal vaccine introduction is critically important to provide baseline and ensure data representativeness across different regions in Indonesia for vaccine impact evaluation in the future.
Methods
We collected nasopharyngeal swabs from children <5 in Wakatobi, Sulawesi and Kotabaru, Kalimantan from October 2018 – April 2019. S. pneumoniae was identified by optochin-susceptibility and bile-solubility test and serotyped using multiplex PCR.
Results
We isolated 305/499 (61%) of S. pneumoniae in Wakatobi and 180/399 (45%) in Kotabaru. 65% of pneumococcus in Wakatobi were PCV13-types. The rate was lower in Kotabaru with 46%. Most common serotypes in Kotabaru were 6A/B, 15B/C and 19F with 18%, 17% and 16%. 6A/B, 23F and 19F were the most common in Wakatobi with 29%, 16% and 9% respectively. Cigarette smoke exposure was associated to colonization in Kotabaru with OR 1.87. Rhinorrhoea and use of kerosene and wood as fuel for cooking were associated to colonization in Wakatobi with OR 2.0 (95%CI 1.3 – 3.0), 3.6 (95%CI 1.4 – 9.0) and 4.3 (95%CI 1.7 – 10.9) respectively.
Conclusions
Different serotype distribution and risk factors were shown despite both communities were the same tribe (Bajau). This result provides baseline data representing indigenous population in Indonesia.
Presenter of 1 Presentation
SEROTYPE DISTRIBUTION AND ANTIMICROBIAL SUSCEPTIBILITY OF STREPTOCOCCUS PNEUMONIAE CARRIED BY HEALTHY BAJAU CHILDREN UNDER 5 YEARS OF AGE IN KOTABARU, SOUTH KALIMANTAN (ID 395)
Abstract
Background
The indigenous Bajau people (Sea Nomads) have lived an entirely marine-dependent existence and renowned for their extraordinary breath-holding abilities. Kotabaru in South Kalimantan is one of the island inhabited by Bajau people. We investigated nasopharyngeal carriage of S. pneumoniae in healthy Bajau children <5 in Kotabaru, South Kalimantan, Indonesia.
Methods
Serotyping was performed using Conventional multiplex PCR. Antimicrobial susceptbility test using disk diffusion and microdulution were employed to all pneumococcal and MDR isolates, respectively.
Results
We found 45% (180/399) of S. pneumoniae. Cigarette exposure contributed to colonization of S. pneumoniae (OR = 1,87). The most common serotypes were 6A/6B (18%), 15B/15C (17%) and 19F (16%). We identified 46% (86/185) was vaccine-type and 40% as non-vaccine type (74/185). The majority of 18% (34/185) defined as Multidrug Resistant pneumococcus were 19F serotype (74%), followed by 6A/6B (9%), 19A (9%), 14 (6%), and 23F (3%). Tetracycline (89%), trimethoprim/sulfamethoxazole (79%), cefuroxime (76%), azithromycin (76%) and erythromycin (76%) showed high prevalence of resistance among MDR isolates with MICs90 were 8, 4/76, >4, and >2 μg/mL, respectively.
Conclusions
Almost half of the identified serotype were vaccine type. All of the multidrug resistant isolates were vaccine type and dominated by 19F. Serotype 15B/15C was the second most common serotype.