PD036 - SINGLE NUCLEOTIDE POLYMORPHISMS OF VITAMIN D RECEPTOR AND VITAMIN D BINDING PROTEIN (VDBP), BUT NOT VITAMIN D AND VDBP SERUM LEVELS, MAY AFFECT SUSCEPTIBILITY TO VIRAL INFECTIONS IN INFANCY (ID 1255)
Abstract
Backgrounds:
The role of Vitamin D in innate and adaptive immunity has been recently demonstrated. The purpose of this study was to explore the potential association of genetic variances in vitamin D pathway, 25(OH)Vitamin D and vitamin D binding protein (VDBP) serum levels and infections in infancy.
Methods
Τhis prospective case-control study included infants 0-24 months with infection and age-matched controls. The single nucleotide polymorphisms of vitamin D receptor (VDR) (BsmI, FokI, ApaI, TaqI), VDBP (rs7041, rs4588) and CYP27B1(rs10877012) were genotyped by polymerase chain reaction and restriction fragment length polymorphism analysis. Serum 25(OH)Vitamin D and VDBP levels were determined using Enzyme-linked Immunosorbent Assay (ELISA). Statistical analysis was conducted with two-tailed Fisher exact, Mann-Whitney and Kruskal-Wallis tests.
Results:
In total 132 infants were enrolled, of whom 40 with bacterial and 52 with viral infection, and 40 healthy controls. ΤaqIwas more frequent in infants with viral infection compared to controls (p= 0.03, OR 1.96, 95% CI 1.1-3.58). Moreover, Gc1F was more frequent in the control group compared to infants with viral infection (p=0.007, OR 2.7, 95%CI 1.3-5.6). No significant differences were found regarding the genetic profile for VDR and VDBP in infants with bacterial infection compared to controls and regarding CYP27B1 (rs10877012) between the studied groups. Serum 25(OH)Vitamin D and VDBP levels did not differ significantly between infants with viral or bacterial infection compared to controls neither between infants with different VDR and VDBP genotypes.
Conclusions/Learning Points:
In this study we demonstrated that genetic variances in vitamin D pathway, but not serum 25(OH)D and VDBP levels, may modulate susceptibility to viral infections in infancy. Our findings further elucidate genetic susceptibility to viral infections and detection of VDR and VDBP genetic profile might help determine high-risk infants.
