Dmitriy Labunskiy, Russian Federation
Presenter of 2 Presentations
COMPLEMENT AS HUMORAL IMMUNITY PARAMETER IN EXPERIMENTAL STRESS
Abstract
Background and Aims
Humoral immune reaction, including complement activation plays important role in mechanisms of stress.
Methods
Quantification of complement levels is based on titration of 50% hemolysis. The phenomenon of complement dependent lysis of sheep erythrocytes loaded with antibodies arises. In accordance with the international standard, such an amount is taken as a unit of complement activity that causes hemolysis of 50% of sensitized red blood cells under standard conditions. In the presence of a constant dose of antigen, a reverse serum titer is determined, i.e., a serum dilution is selected at which such an amount of complement is bound that 50% inhibition of hemolysis occurs.
Results
A study of the effect of antidepressants on the immune responses of stressed animals showed that intraperitoneal administration of amitriptyline and paroxetine in the form of monotherapy under stress is accompanied by even greater changess of immunological parameters of blood mice than with saline. At the same time, despite unidirectional changes in immune responses, amitriptyline caused more pronounced dysfunctional immune disorders than paroxetine: it significantly suppressed the phagocytic and metabolic activity of neutrophils, contributed to the accumulation of large, medium and small fractions of circulating immune complexes (up to 12.4 + 0.5, 37.5 + 5.3, 143.4 + 9.2, respectively). The disturbed ratio of leukocyte subpopulations in the leukoformula, caused by stress, persisted with amitriptyline monotherapy - there was an increase of segmented neutrophils, lymphopenia.
Conclusions
Use of psychoactive drugs such as amitriptyline and paroxetine seems plays significant role in stress inducted changes of innate immune responses.
STUDY OF THE CONCENTRATION OF SMALL FRACTION IMMUNE COMPLEXES IN THE TISSUES OF EXPERIMENTAL WHITE MICE UNDER CONDITIONS OF IMMOBILIZATION STRESS
Abstract
Background and Aims
Immobilization stress plays a crucial role in autoimmune reactions. Smll fractions of circular immune complexes (CIC) reflect these physiological changes.
Methods
The study of humoral immune reactions was carried out with the participation of 160 white mice of both sexes, weighing 18-23 g. Humoral immunity indicators were determined by conventional methods: quantitative determination of murine cytokines - IL-1b and IL-10 in blood serum was carried out by enzyme-linked immunosorbent assay using test kits manufactured by Bender MedSystems CJSC BioChemMak (Moscow); components of the complement system were studied by the hemolytic method of E. Kabat and M. Mayer.
Results
The restriction of the motor regime of animals in combination with injection stress led to an imbalance of humoral immune responses. The degree of change in the studied parameters depended on the duration of the stressful effect. After 1 day, IP plasma concentrations of pro-inflammatory and anti-inflammatory cytokines, components of the complement system, IC concentration in the tissues of internal organs did not change relative to the group of intact animals.
By the 5th day of immobilization, pronounced changes in indicators of humoral immunity persisted. There was a decrease in the complementary activity of blood serum mainly due to the C1, C4 and C5 components of complement. A significant increase in the formation ofCIC) was observed: the number of large CICs increased by an order of magnitude, medium and small by 3 and 2 times, respectively.
Conclusions
CIC from different organs were found in different concentrations, which show various scenarios of autoimmune responces.