COMPLEMENT AS HUMORAL IMMUNITY PARAMETER IN EXPERIMENTAL STRESS
- Dmitriy Labunskiy, Russian Federation
Abstract
Background and Aims
Humoral immune reaction, including complement activation plays important role in mechanisms of stress.
Methods
Quantification of complement levels is based on titration of 50% hemolysis. The phenomenon of complement dependent lysis of sheep erythrocytes loaded with antibodies arises. In accordance with the international standard, such an amount is taken as a unit of complement activity that causes hemolysis of 50% of sensitized red blood cells under standard conditions. In the presence of a constant dose of antigen, a reverse serum titer is determined, i.e., a serum dilution is selected at which such an amount of complement is bound that 50% inhibition of hemolysis occurs.
Results
A study of the effect of antidepressants on the immune responses of stressed animals showed that intraperitoneal administration of amitriptyline and paroxetine in the form of monotherapy under stress is accompanied by even greater changess of immunological parameters of blood mice than with saline. At the same time, despite unidirectional changes in immune responses, amitriptyline caused more pronounced dysfunctional immune disorders than paroxetine: it significantly suppressed the phagocytic and metabolic activity of neutrophils, contributed to the accumulation of large, medium and small fractions of circulating immune complexes (up to 12.4 + 0.5, 37.5 + 5.3, 143.4 + 9.2, respectively). The disturbed ratio of leukocyte subpopulations in the leukoformula, caused by stress, persisted with amitriptyline monotherapy - there was an increase of segmented neutrophils, lymphopenia.
Conclusions
Use of psychoactive drugs such as amitriptyline and paroxetine seems plays significant role in stress inducted changes of innate immune responses.