Li-Juan Song, China

Shanxi University of Traditional Chinese Medicine The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Chinese Medicine, and Scientific and Technological Innovation Team of Integrated Chinese and Western Medicine for the P
Lijuan Song, born in November 1985, is an associate professor and Deputy Director of Department of Western Medicine at Shanxi University of Traditional Chinese Medicine. She holds a doctorate in physiology from Capital Medical University and is a post-doctor of Shanxi Medical University. She is engaged in the basical |and clinical research and teaching work of Brain Disease by integrated Chinese and Western Medicine. She has presided over several important projects including National Natural Science Fundation of China and China Postdoctoral Science Fundation. She has published more than 30 academic papers and made several reports in international academic conferences. As the first editor-in-chief, she has published two books, the Foundation and Clinical Practice of Modern Chinese Medicine and the Scheme of Diagnosis and Treatment of Neurological Diseases. In addition，She has hold 3 related patents.

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EFFECTS OF SAFFLOWER YELLOW PIGMENT ON THE SPLEEN CELLS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS MICE

Session Name
PS50 - THE INVOLVEMENT OF AUTOIMMUNITY IN CNS
Session Type
PARALLEL SESSIONS
Date
31.05.2021, Monday
Session Time
13:30 - 15:30
Room
HALL D
Lecture Time
15:10 - 15:20
Presenter
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Abstract

Background and Aims

To investigate the efficacy and possible mechanism of safflower yellow pigment (SY) on spleen cells in experimental autoimmune encephalomyelitis (EAE) mice.

Methods

Female C57BL/6 mice were induced with myelin oligodendrocyte glycoprotein 35-55 to establish an EAE model. Splenocytes were prepared on day 9 after immunization, and cultured with/ without safflower yellow pigment. The proliferation of splenocytes were detected by CCK-8 method and CFSE method. TNF-α, IL-1β, IFN-γ, IL-17 and IL-4 in the supernatant were detected by ELISA. Flow cytometry was used to detect the changes of M1 and M2 macrophage phenotypes, and the Griess method was used to detect the NO content in the supernatant.

Results

When the concentration of SY was 26.24mg/L, the proliferation rate of spleen cells could be increased (P<0.01), and the inflammatory factors TNF-α, IL-1β, IFN-γ, IL-17 were inhibited and the expression of antiinflammatory factor IL-4 in the supernatant were decreased (P<0.01). The ratio of proinflammatory macrophages in spleen was increased (P<0.01, P<0.05 respectively). NO content was decreased in the cultured supernatant (P<0.01).

Conclusions

The effectiveness of SY on spleen cells of EAE mice is related to the regulation of cytokine levels and the regulation of the ratio of proinflammatory and anti-inflammatory macrophages in the spleen. In addition, SY can also reduce the NO content. (Astragalus Resource Industrialization and Industrial Internationalization Cooperative Innovation Center Project of Shanxi Province, HQXTCXZX2016-028)

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