EFFECTS OF SAFFLOWER YELLOW PIGMENT ON THE SPLEEN CELLS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS MICE
- Li-Juan Song, China
Abstract
Background and Aims
To investigate the efficacy and possible mechanism of safflower yellow pigment (SY) on spleen cells in experimental autoimmune encephalomyelitis (EAE) mice.
Methods
Female C57BL/6 mice were induced with myelin oligodendrocyte glycoprotein 35-55 to establish an EAE model. Splenocytes were prepared on day 9 after immunization, and cultured with/ without safflower yellow pigment. The proliferation of splenocytes were detected by CCK-8 method and CFSE method. TNF-α, IL-1β, IFN-γ, IL-17 and IL-4 in the supernatant were detected by ELISA. Flow cytometry was used to detect the changes of M1 and M2 macrophage phenotypes, and the Griess method was used to detect the NO content in the supernatant.
Results
When the concentration of SY was 26.24mg/L, the proliferation rate of spleen cells could be increased (P<0.01), and the inflammatory factors TNF-α, IL-1β, IFN-γ, IL-17 were inhibited and the expression of antiinflammatory factor IL-4 in the supernatant were decreased (P<0.01). The ratio of proinflammatory macrophages in spleen was increased (P<0.01, P<0.05 respectively). NO content was decreased in the cultured supernatant (P<0.01).
Conclusions
The effectiveness of SY on spleen cells of EAE mice is related to the regulation of cytokine levels and the regulation of the ratio of proinflammatory and anti-inflammatory macrophages in the spleen. In addition, SY can also reduce the NO content. (Astragalus Resource Industrialization and Industrial Internationalization Cooperative Innovation Center Project of Shanxi Province, HQXTCXZX2016-028)