Author Of 1 Presentation
P0523 - Genetic polymorphisms associated with multiple sclerosis and atherosclerosis (ID 745)
Abstract
Background
Subclinical atherosclerosis has been inconsistently investigated in MS. Several single-nucleotide polymorphisms (rs877087 (Ryanodine Receptor 3 gene), rs429358 (Apolipoprotein E gene), rs2229116 (Ryanodine Receptor 3 gene), rs5498 (Intercellular Adhesion Molecule 1 gene), rs763780 (Interleukin 17F gene), rs2275913 (Interleukin 17a gene), rs7412 (Apolipoprotein E gene), rs1800795 (Interleukin 6 gene)) have been linked to MS and atherosclerosis.
Objectives
Multiple sclerosis (MS) and atherosclerosis are both inflammatory diseases. The aim of the current study was to determine whether patients with MS have increased risk for subclinical atherosclerosis and to quantify the magnitude of the association between genetic polymorphisms related to atherosclerosis and MS. The goal was also to compare the frequency of genetic polymorphisms between the group of patients with MS and the group of healthy controls.
Methods
We enrolled 97 relapsing-remitting MS (RR MS) patients and 36 healthy controls. This study was approved by the Regional Ethics Committee and written informed consent was obtained from all patients. DNA was extracted from peripheral blood mononuclear cells. Genotyping was performed using an Illumina GSA v1.0 BeadChip. Data analysis was performed using PLINK v1.9 and using a targeted SNP approach. Intima media thickness (IMT) in the common carotid arteries (CCA) was obtained from three digitalized ultrasound images from the same sections. Interleukin-6 (IL-6) and high-sensitivity C-reactive protein (hs-CRP) serum levels were also determined.
Results
Association analysis has shown statistically significant higher frequency of APOE rs7412 T allele in patients (0.26) in comparison with healthy controls (0.06) (adj. P=0.005; OR: 5.53). Subsequently, logistic regression analysis was performed in order to adjust for age, sex, BMI, smoking, CCA IMT, IL-6 and hs-CRP levels. In spite of adjustment, association of SNP rs7412 remained statistically significant (P=7.02×10-4; OR: 8.63; CI95: 2.5-30). Furthermore, analysis of only patients has shown statistically significant association of IL-6 serum levels and IL17A rs2275913 (P=0.027; OR: 1.95). Individuals with AA genotype had higher mean values of IL-6 serum levels (5.6±2.5) in comparison with individuals with AG (4.8±1.9) or GG (4.2±2.0) genotype.
Conclusions
Findings of the present study suggest higher prevalence of some genetic polymorphisms associated with atherosclerosis in RR MS patients group compared to healthy controls.