Marine macroalgae
The biosynthesized SW-SeNPs was characterized using Transmission Electron Microscopy (TEM), Zeta potential analysis, Fourier Transform-Infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). The anticancer effect of SW-SeNPs against A549 cells was determined. Various parameters such as cell viability, reactive oxygen species (ROS) production, DNA fragmentation, cell cycle progression and apoptosis were studied. Also, the level of p53, p-Akt and caspase-3 were determined using flow cytometry.
SW-SeNPs was found to be in the range of 20 - 40 nm size with crystalline nature. The negative zeta potential of SW-SeNPs and FT-IR analysis confirmed the surface binding of bioactive components present in S. wightii in the SW-SeNPs. SW-SeNPs was studied for its anticancer potential against A549 cells. SW-SeNPs affected the A549 cells viability in dose-dependent manner. SW-SeNPs treatment increased the ROS generation and led to DNA fragmentation in A549 cells. Also, the cells were arrested in G2/M phase which ultimately induced apoptosis of the A549 cells through caspase-3 activation. The molecular mechanism of SW-SeNPs-induced A549 cells apoptosis was found to be through overproduced ROS-mediated activation of p53 and AKT signal pathways.
Altogether, the present study demonstrated the anticancer therapeutic effect of SW-SeNPs against lung cancer.
The authors.
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All authors have declared no conflicts of interest.