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O093 - EXPLORING THE DIFFERENCE OF NASOPHARYNGEAL MICROBIOME IN COLONIZED STREPTOCOCCUS PNEUMONIAE AND INVASIVE PNEUMOCOCCAL DISEASE: AN INSIGHT TO THE DEVELOPMENT OF INVASIVE PNEUMOCOCCAL DISEASE (ID 617)
Abstract
Background
Streptococcus pneumoniae (pneumococcus), is an opportunistic pathogen that commonly colonizes the upper airway asymptomatically. It is responsible for life-threatening invasive pneumococcal disease (IPD) around the world. Nasopharyngeal microbiome (NPM) can be one of the important factors which influence the penetration to host tissues and progression of IPD. Differences in the NPM in colonized pneumococcus (CP) and IPD may unveil the key factors in development of IPD as CP can progress in IPD.
Methods
NPM (V4-region of 16S-rRNA genes sequenced via next-generation sequencing) from the children corresponding to two groups (CP and IPD) were collected from recent studies. Phylogenetic analysis, taxonomic annotation, diversity analysis and differential abundance of taxa were conducted through FastTree, q2-feature-classifier, q2-diversity module in QIIME2 and LEfSe respectively.
Results
High Faith’s phylogenetic-diversity and low Pielou’s evenness were observed in IPD-group (p-value<0.001) that may be the pattern in NPM which favors the IPD progression (Figure-1A-B). Significant difference was also observed in the permutational multivariate analysis of variance (PERMANOVA) between the groups (Figure-1). Finally, 30 differentially abundant taxa between the groups were identified (Figure-1). High abundance of streptococcus ovis and Streptococcus equi was found in IPD which may favor the IPD development. Similarly, Salmonella Infantis and Streptococcus salivarius were found in CP which may suppress the development of IPD (Figure-1J-K).
Conclusions
Patterns in microbiome diversity and abundance of species that may favor/suppress the IPD were observed. Importantly, confounding factors such as diverse populations and age groups were present in the study and further studies may be required to confirm the findings.