Hema Sori,
Author Of 1 Presentation
DELIVERY OF PNEUMOCOCCAL ANTIGENS USING POLYMERIC NANOPARTICLES AND ITS IMPLICATIONS ON IMMUNOGENICITY (ID 820)
Abstract
Background
Commercially licensed pneumococcal vaccines provide effective immunity against specific serotypes but have their own limitations. They fail to induce memory, require multiple doses and lead to serotype replacement. Pneumococcal Capsular polysaccharides (PCP) serve as major virulence factors, but are weakly immunogenic. In order to make this subunit based vaccine more effective; Poly lactide polymeric particles entrapping pneumococcal polysaccharide and its homologous conserved surface protein SP0845 were formulated and were immunologically evaluated.
Methods
PCP type 1, type 5 and recombinant SP0845 protein was entrapped in polymeric nanoparticles using double emulsion solvent evaporation method. In vivo studies were performed in BALB/c mice to evaluate cytokines and antibody response. ELISPOT assay was performed to enumerate antigen specific antibody secreting splenic cells in immunized mice.
Results
Pneumococcal capsular polysaccharide type 1 and 5 entrapped in PLA polymeric particles induced higher IgM and IgG memory antibody response than their soluble counterparts. Immunogenicity of SP0845 protein (tag free) was enhanced by delivering it using poly-lactide polymeric nanoparticles. Number of PCP type 1 specific IgG secreting cells detected in the spleen of mice immunized with PCP-1 entrapped nanoparticles were higher as compared to its soluble form.
Conclusions
Entrapment of pneumococcal antigens in polymeric particles significantly improves their antibody response.