Kim Anh Le,
Author Of 1 Presentation
SEROTYPE DISTRIBUTION, BIOFILM FORMATION, AND ANTIBIOTIC SUSCEPTIBILITY PATTERN OF STREPTOCOCCUS PNEUMONIAE ISOLATED FROM HOSPITALIZED PEDIATRIC ACUTE RESPIRATORY CASES IN NHA TRANG, VIETNAM (ID 1115)
Abstract
Background
Streptococcus pneumoniae forms biofilms during asymptomatic carriage, which promotes persistence in the niche, intraspecies, and interspecies communication, and contributes to the development of the invasive pneumococcal disease. In this study, we sought to evaluate biofilm formation and its association with serotype pattern and antibiotic susceptibility of S. pneumoniae strains.
Methods
We investigated 83 S. pneumoniae strains isolated from nasopharyngeal swab samples collected from children under five years of age with acute respiratory infections hospitalized at Khanh Hoa General Hospital between October to December 2015. Biofilm formation was determined by the ability of cells to adhere to the walls and base of 96-well polystyrene dishes. Multiplex PCR assay was used for serotyping, and the microbroth dilution method was performed for 16 antibiotic susceptibility testing.
Results
Biofilm formation occurred in 14 (16.9%) strains of vaccine serotypes. Among these 14.3%, 85.7%, 92.8% and 100% of the isolates had high MICs for penicillin group (≥ 8 µg/mL), tetracycline (≥ 2 µg/mL), clindamycin and erythromycin (≥ 2 µg/mL), and cefaclor (≥ 4 µg/mL) respectively and most of the isolates were PCV13 vaccine serotypes.
Conclusions
We found serotype 19F is widely prevalent in Vietnamese children. Biofilm formation was not found significantly associated with drug susceptibility.