Yurika P. Diah, Indonesia
dr. Soedono General Hospital Madiun East Java Clinical MicrobiologyAuthor Of 1 Presentation
ANALYSIS OF ERMB AND MEFA GENES FROM NON-SUSCEPTIBLE ERYTHROMYCIN STREPTOCOCCUS PNEUMONIAE ISOLATES IN INDONESIA (ID 564)
Abstract
Background
Macrolide resistance of Streptococcus pneumoniae has already increased in worldwide. In Asia, >70% of clinical S. pneumoniae isolates was reported as erythromycin resistant. ErmB and mefA genes existence plays a role in resistance mechanism by modification of drug binding site and active efflux of the cell. This study is aimed to analyze macrolide resistant genes (ermB and mefA) associated with prevalence of non-susceptible erythromycin S. pneumoniae isolates in Indonesia.
Methods
Seventy isolates of S. pneumoniae were processed for antimicrobial susceptibility testing according to CLSI. The ermB and mefA genes were detected using duplex PCR. Association between these genes and erythromycin resistance rate was analyzed using Fisher test.
Results
A total of 47 (67%) isolates were nonsusceptible to erythromycin. ErmB was detected in 24 (34.2%) isolates and mefA was detected in 50 (71.4%) isolates. Isolates having both ermB and mefA were 34 (48.5%) isolates. There was association between erythromycin resistance with existence of mefA gene (P < 0.001), ermB (P = 0,045) and both (P = 0,036). The most common resistance isolates were serotype 19F (38,2%) and 23F (31,9%).
Conclusions
The existence of mefA and ermB seems to be associated with erythromycin non-susceptible of S. pneumoniae where mefA is the most associated to resistance.