Jesus Reine, United Kingdom
Liverpool School of Tropical Medicine Clinical SciencesAuthor Of 6 Presentations
NASAL PNEUMOCOCCAL COLONIZATION ASSOCIATES WITH ALLERGIC INFLAMMATION IN CHILDREN (ID 779)
- Simon P. Jochems, Netherlands
- Jesus Reine, United Kingdom
- Wouter A. De Steenhuijsen Piters, Netherlands
- Elissavet Nikolaou, United Kingdom
- Carla Solorzano, United Kingdom
- Sherin Pojar, United Kingdom
- Elena Mitsi, United Kingdom
- Helen Hill, United Kingdom
- Debby Bogaert, United Kingdom
- Paul S. McNamara, United Kingdom
- Daniela M. Ferreira, United Kingdom
THE MUCOSAL AND SYSTEMIC ANTIBODY REPERTOIRE FOLLOWING EXPERIMENTAL PNEUMOCOCCAL CHALLENGE IN HEALTHY ADULTS (ID 993)
- Joseph J. Campo, United States of America
- Carla Solorzano, United Kingdom
- Esther L. German, United Kingdom
- Jesus Reine, United Kingdom
- Sherin Pojar, United Kingdom
- Elissavet Nikolaou, United Kingdom
- Elena Mitsi, United Kingdom
- Timothy Q. Le, United States of America
- Xiaowu Liang, United States of America
- Daniela M. Ferreira, United Kingdom
Abstract
Background
In the experimental human pneumococcal challenge (EHPC) model, healthy adults are intranasally inoculated with a pneumococcal challenge strain. A panproteome Streptococcus pneumoniae array, containing over 2,600 pneumococcal proteins, was used to screen systemic and mucosal antibodies from EHPC volunteers.
Methods
IgG and IgA responses were profiled in a cohort of 150 volunteers challenged with serotype 6B pneumococci, half of whom were susceptible to experimental colonization and the other half remained protected. Serum and nasal wash samples collected pre- and post-EHPC were probed on the S. pneumoniae panproteome microarray with simultaneous detection of IgA and IgG binding.
Results
Hundreds of pneumococcal proteins were reactive with serum and nasal wash IgG and IgA. IgA- and IgG-reactive proteins showed high levels of overlap. However, over 200 proteins were reactive only with IgG. Antibodies against numerous proteins significantly increased following challenge. Unsupervised clustering showed subject-specific stability of antibody profiles in serum, but independently grouped profiles in nasal wash samples collected before and after challenge. No specific profile differences were observed in pre-EHPC samples between susceptible and protected groups, but the response to EHPC showed unique antigen reactivity patterns.
Conclusions
A single encounter with pneumococci can elicit broad changes in mucosal and systemic antibody responses to pneumococcal proteins.
CHARACTERIZING THE IMMUNE RESPONSE TO EXPERIMENTAL PNEUMOCOCCAL CARRIAGE IN OLDER ADULTS (ID 682)
- Dessi Loukov, United Kingdom
- Fernando Marcon, United Kingdom
- Hugh Adler, United Kingdom
- Simon P. Jochems, Netherlands
- Jesus Reine, United Kingdom
- Carla Solorzano, United Kingdom
- Esther L. German, United Kingdom
- Sherin Pojar, United Kingdom
- Elena Mitsi, United Kingdom
- Elissavet Nikolaou, United Kingdom
- Stephen Gordon, Malawi
- Jamie Rylance, United Kingdom
- Daniela M. Ferreira, United Kingdom
MUTATIONS OF STREPTOCOCCUS PNEUMONIAE BIOSYNTHESIS GENES INFLUENCE EPITHELIAL MICRO-INVASION AND THE INNATE-EPITHELIAL CELL RESPONSE IN VITRO AND IN AN EXPERIMENTAL HUMAN PNEUMOCOCCAL CHALLENGE MODEL (ID 872)
- Caroline M. Weight, United Kingdom
- José-Afonso Guerra-Assuncao, United Kingdom
- Elisa Ramos-Sevillano, United Kingdom
- Annie Blizard, United Kingdom
- Jesus Reine, United Kingdom
- Madhad Noursadeghi, United Kingdom
- Daniela M. Ferreira, United Kingdom
- Jeremy Brown, United Kingdom
- Robert S. Heyderman, United Kingdom
ASSESSING THE PROTECTION OF LIVE ATTENUATED PNEUMOCOCCAL VACCINES AGAINST EXPERIMENTAL HUMAN PNEUMOCOCCAL CARRIAGE (ID 1175)
- Helen Hill, United Kingdom
- Annie Blizard, United Kingdom
- M Gautan,
- Emily Gibbons,
- Ashleigh Howard, United Kingdom
- Angie Hyder-Wright,
- Simon P. Jochems, Netherlands
- Dessi Loukov, United Kingdom
- Daniella McLenaghan, United Kingdom
- Elena Mitsi, United Kingdom
- Elissavet Nikolaou, United Kingdom
- Sherin Pojar, United Kingdom
- Jesus Reine, United Kingdom
- Ryan Robinson,
- Elisa Ramos-Sevillano, United Kingdom
- Carla Solorzano, United Kingdom
- Stephen Gordon, Malawi
- Jeremy Brown, United Kingdom
- Daniela M. Ferreira, United Kingdom
Abstract
Background
We have previously reported that pneumococcal colonization increases lung and systemic protective immune responses. Live attenuated bacteria may be a good strategy for adult vaccination, particularly for those with chronic lung disease.
Methods
Single-blind RCT (superiority design). Healthy adults (age 18 to 50 years) were randomised 1:1:1:1 to be nasally vaccinated twice (two weeks interval) with either saline, wild-type 6B (BHN418) or a genetically modified strain (A1 or A2 – double mutants constructed on the BHN418 backbone). After 6 months, participants were challenged with wild-type 6B to assess protection against colonization acquisition.
Results
No Serious Adverse Events reported. 202 participants were assessed for eligibility, 148 randomised and 126 completed the study as per modified intention to treat. Rates of carriage acquisition following challenge with wild-type 6B were as follow: saline (negative control): 17/32 (47%), wild-type 6B (positive control): 9/31 (29%), A1: 9/30 (30%) and A2: 16/33 (49%).
Conclusions
Protection following nasal inoculation with attenuated strain (A1) was comparable to 6B wild-type. Attenuation in virulence by genetic modification may allow the development and safe use of live nasal vaccines whilst providing broad coverage against pneumococcal infection and improved lung immunity.
PRECEDING PNEUMOCOCCAL COLONIZATION MODULATES NASAL AND LUNG MUCOSAL IMMUNE RESPONSES TO LIVE ATTENUATED INFLUENZA VACCINATION IN ADULTS (ID 323)
- Elena Mitsi, United Kingdom
- Beatriz Carniel, United Kingdom
- Fernando Marcon, United Kingdom
- Jamie Rylance, United Kingdom
- Jesus Reine, United Kingdom
- Edessa Negera, United Kingdom
- Andrea Collins, United Kingdom
- Elissavet Nikolaou, United Kingdom
- Sherin Pojar, United Kingdom
- Carla Solorzano, United Kingdom
- Debby Bogaert, United Kingdom
- Stephen Gordon, Malawi
- Helder Nakaya, Brazil
- Simon P. Jochems, Netherlands
- Daniela M. Ferreira, United Kingdom