Fan Zhang,
Author Of 1 Presentation
THE ROLE OF CARRIER-SPECIFIC T-HELPER CELLS IN FACILITATING PRIMING AND BOOST/RECALL OF ANTIBODY RESPONSE TO POLYSACCHARIDE ANTIGEN (ID 1022)
Abstract
Background
Coupling polysaccharides to protein-carriers enables generation of robust anti-polysaccharide response in a T-dependent manner. One mechanism is via engagement of polysaccharide-specific T-helper cells; it is unclear if carrier-specific T-helper cells (Tcarrier) play any role. We study the role of Tcarrier in priming and boost/recall of antibody response.
Methods
Rag1-/- mice were adoptively transferred with B cells from naïve mice or mice primed with 5-valent Multiple Antigen Presenting System (MAPS) PS-protein vaccine, with or without CD4+ T cells from naïve or carrier-primed mice, prior vaccination with 5-valent MAPS. Serum anti-polysaccharide IgG was compared between groups pre- and post-vaccination. Separately, wild-type mice were primed with type4-pneumolysoid MAPS and exposed to heat-killed wild-type or pneumolysin-/- type4 pneumococci for comparison of recalled anti-type4 polysaccharide response.
Results
For 3/5, and 4/5 polysaccharides, carrier-primed T cells, compared to unprimed T cells, enhanced vaccine-induced anti-polysaccharide IgG production by naïve B cells (priming) or memory B cells (recall), respectively. Priming mice with type4-pneumolysoid MAPS vaccine resulted in significantly higher recalled anti-type4 polysaccharide IgG after exposure to a pneumolysin-producing vs. pneumolysin-deficient strain.
Conclusions
Tcarrier facilitates priming and boosting of anti-polysaccharide response. Using pneumococcal proteins as vaccine carriers may lead to higher antibody level recall during active pneumococcal infection or colonization.
Presenter of 1 Presentation
THE ROLE OF CARRIER-SPECIFIC T-HELPER CELLS IN FACILITATING PRIMING AND BOOST/RECALL OF ANTIBODY RESPONSE TO POLYSACCHARIDE ANTIGEN (ID 1022)
Abstract
Background
Coupling polysaccharides to protein-carriers enables generation of robust anti-polysaccharide response in a T-dependent manner. One mechanism is via engagement of polysaccharide-specific T-helper cells; it is unclear if carrier-specific T-helper cells (Tcarrier) play any role. We study the role of Tcarrier in priming and boost/recall of antibody response.
Methods
Rag1-/- mice were adoptively transferred with B cells from naïve mice or mice primed with 5-valent Multiple Antigen Presenting System (MAPS) PS-protein vaccine, with or without CD4+ T cells from naïve or carrier-primed mice, prior vaccination with 5-valent MAPS. Serum anti-polysaccharide IgG was compared between groups pre- and post-vaccination. Separately, wild-type mice were primed with type4-pneumolysoid MAPS and exposed to heat-killed wild-type or pneumolysin-/- type4 pneumococci for comparison of recalled anti-type4 polysaccharide response.
Results
For 3/5, and 4/5 polysaccharides, carrier-primed T cells, compared to unprimed T cells, enhanced vaccine-induced anti-polysaccharide IgG production by naïve B cells (priming) or memory B cells (recall), respectively. Priming mice with type4-pneumolysoid MAPS vaccine resulted in significantly higher recalled anti-type4 polysaccharide IgG after exposure to a pneumolysin-producing vs. pneumolysin-deficient strain.
Conclusions
Tcarrier facilitates priming and boosting of anti-polysaccharide response. Using pneumococcal proteins as vaccine carriers may lead to higher antibody level recall during active pneumococcal infection or colonization.