Jakub Kopycinski (United Kingdom)

University of Oxford Nuffield Dept of Medicine

Presenter of 1 Presentation

 Conference Calendar
Clinical Research / Therapeutic Vaccines – Clinical Aspects ePoster

INVESTIGATION OF THE RELATIONSHIP BETWEEN HR-HPV-SPECIFIC T-CELL RESPONSES AND HR-HPV DNA IN HEALTHY WOMEN AND WOMEN WITH CERVICAL ABNORMALITIES IN A LONGITUDINAL STUDY (ID 790)

Session Date
07/21/2020
Session Time
10:00 - 17:00
Room
ePoster
Session Type
Poster Viewing - 20-24 July
Session Name
Clinical Research / Therapeutic Vaccines – Clinical Aspects
Lecture Time
10:03 - 10:04
Presenter
Authors

Abstract

Introduction

Cell-mediated immune responses play a crucial role in regression of HPV lesions and clearance of infections. However, studies evaluating the role of T cell responses to specific proteins have produced conflicting results, likely reflecting the diverse methods used and predominance of cross-sectional analyses.

Methods

We established a prospective longitudinal cohort of women aged 16-55 years to study the relationship between detection of T-cell responses to early proteins and detection of hrHPV DNA. Cohort 1 comprises asymptomatic sexually active women aged 16-24. Cohort 2 comprises women aged 25-55 undergoing colposcopy, with mild, moderate or severe dyskaryosis. Women are followed for 12 months and provide a blood sample and self-taken vaginal swab at 4-monthly (Cohort 1) or 6-monthly (Cohort 2) intervals. T cell responses to all hrHPV early proteins are quantified by IFNγ Elispot and flow cytometry using overlapping 15-mer peptides based on HPV16, HPV52 and selected conserved sequences across 5 hrHPV genotypes.

Results

144 women were enrolled in the study: 107 in Cohort, mean age 21y; 37 in Cohort 2, mean age 32y. Baseline data are reported. Prior prophylactic vaccination was reported in 88% Cohort 1 and 21% Cohort 2. HPV DNA was detected in 27 (25%) Cohort 1 and 31 (91%) Cohort 2. hrHPV isolates were non-16/18 types in 100% Cohort 1 and 82% Cohort 2. HPV16/18 were also detected in 21% Cohort 2. T-cell responses to E1/E2 or E6/E7 peptides (HPV52 > HPV16) were detected in 22/103 (21%) Cohort 1 and 15/31 (48%) Cohort 2. Overall, E1/E2 was most frequently targeted.

Conclusions

There was no consistent relationship between baseline hrHPV DNA prevalence and T-cell reactivity. Our data suggest that transient infections infrequently elicit T-cell responses. Longitudinal analysis is directed at determining the phenotype and duration of T-cell responses that are associated with hrHPV clearance or persistence.

Hide