Ayslan C. Brant (Brazil)

Instituto Nacional de Câncer Genetics

Presenter of 2 Presentations

 Conference Calendar
Basic Research / Regulation of Gene Expression ePoster

PREFERENTIAL EXPRESSION OF A SINGLE HPV GENOTYPE IN INVASIVE CERVICAL CARCINOMAS INFECTED BY MULTIPLE HPV GENOTYPES (ID 298)

Session Date
07/21/2020
Session Time
10:00 - 17:00
Room
ePoster
Session Type
Poster Viewing - 20-24 July
Session Name
Basic Research / Regulation of Gene Expression
Lecture Time
10:00 - 10:01
Presenter
Authors

Abstract

Introduction

Infection by multiple HPV genotypes is frequently detected in cervical lesions and is considered a risk factor to carcinogenesis. Although evidences indicate that each HPV genotype infection occurs independently, with limited interaction between different genotypes, the carcinogenic process associated with HPV coinfections is not well established. Here we characterized the HPV genome expression and integration in patients with invasive cervical carcinomas associated with infections by multiple HPV genotypes.

Methods

DNA and RNA extracted from the same biopsy fragment of 19 invasive cervical carcinomas were used, respectively, for: (i) hybridization assay to identify the presence of multiple HPV genotypes, and (ii) for RNA-seq analysis to characterize the expression and integration of the different HPV genotypes.

Results

DNA hybridization assay identified the presence of two to six different HPV genotypes in each biopsy. No preferential coinfection between genotypes belonging to the same HPV species was observed, however, HPV16 infection was detected in 18 of the 19 samples. Simultaneously low-risk and high-risk HPVs infection was observed in four samples. The RNA-seq analysis showed that in 16 samples a single HPV genotype was preferentially expressed, with mean coverage per base >20x in 14 samples and >3x in 2 samples, while the renaming genotypes were low (coverage <0.5x) or not expressed. Additional 3 samples showed low number of HPVs reads (<200), indicating low or no expression of the genotypes (coverage per base <0.3x). Finally, the search for HPV/human chimeric transcripts identified the HPV integration site in 12 samples, which a single sample with two different HPV genotypes simultaneously integrated. The integrated HPV genotype was the one preferentially expressed.

Conclusions

The present study indicates that in invasive cervical carcinomas with multiple HPVs infection a single virus type has its genome preferentially expressed, suggesting that this genotype is responsible for the tumor progression and maintenance.

Hide
Oral Session 7: Host Genomics and Viral Gene Regulation Hall C

HPV18 UTILIZES TWO ALTERNATIVE BRANCH SITES FOR E6*I SPLICING TO PRODUCE E7 PROTEIN (ID 296)

Session Date
07/24/2020
Session Time
12:35 - 13:40
Room
Hall C
Session Type
Basic Science Oral Session
Session Name
Oral Session 7: Host Genomics and Viral Gene Regulation
Lecture Time
13:14 - 13:26
Presenter
Authors

Webcast

[session]
[presentation]
[presenter]
Hide

Moderator of 1 Session

 Conference Calendar
Basic Science Oral Session

01010 - Oral Session 7: Host Genomics and Viral Gene Regulation (ID 25)

Session Type
Basic Science Oral Session
Session Date
07/24/2020
Session Time
12:35 - 13:40
Room
Hall C
Chair(s)

Session Webcast