R. Uchiyama (Isehara, JP)
Tokai University School of Medicine orthopaedicPresenter Of 1 Presentation
P204 - Changes in Platelet Activation Through the Preparation and Freeze-thawing of Platelet Rich Plasma
Abstract
Purpose
Osteoarthritis of the knee (OAK) is a chronic degenerative disease accompanied by damage to the articular cartilage, and no current treatment can completely reverse OAK. Recently, joint treatment with platelet-rich plasma (PRP), an autologous blood product generated by centrifuging whole blood and concentrating platelets, has attracted attention as a point-of-care treatment. PRPs used in clinical practice are usually administered to the affected area immediately after preparation, but PRPs used in research is often cryopreserved. However, platelets are known to become activated by stimuli such as freezing or centrifugation. In this study, we compared PRPs prepared with two different kits to investigate the effects of PRP preparation, and freezing and thawing on platelet activation.
Methods and Materials
PRPs were purified from 60 ml of peripheral blood of OAK patients (n=14, 64.0±10.3 years) using JMS Cell-Aid P-type kit (group J) or Zimmer Biomet APS kit (group Z). Surplus PRPs were frozen at -80°C. Using flow cytometry, the expression of CD62P, a marker for activated platelets, was analyzed in peripheral blood, freshly prepared PRP, and frozen-thawed PRP.
Results
The average CD62P positive rates for platelets in peripheral blood (10.5% in group J and 17.6% in group Z) were not significantly different. The platelet activation rates increased significantly in fresh PRP (15.6% in group J and 26.5% in group Z) and were significantly different between the two groups. The platelet activation rates in frozen-thawed PRP (53.5% in group J and 84.3% in group Z) further increased significantly and were significantly different between the two groups.
Conclusion
Platelets are known to release their contained humoral factors when activated. The content of humoral factors may be different in PRPs with different platelet activation rates, leading to possibly different treatment effects. In the future, we will verify this effect on target cells.
Presenter Of 1 Presentation
P204 - Changes in Platelet Activation Through the Preparation and Freeze-thawing of Platelet Rich Plasma
Abstract
Purpose
Osteoarthritis of the knee (OAK) is a chronic degenerative disease accompanied by damage to the articular cartilage, and no current treatment can completely reverse OAK. Recently, joint treatment with platelet-rich plasma (PRP), an autologous blood product generated by centrifuging whole blood and concentrating platelets, has attracted attention as a point-of-care treatment. PRPs used in clinical practice are usually administered to the affected area immediately after preparation, but PRPs used in research is often cryopreserved. However, platelets are known to become activated by stimuli such as freezing or centrifugation. In this study, we compared PRPs prepared with two different kits to investigate the effects of PRP preparation, and freezing and thawing on platelet activation.
Methods and Materials
PRPs were purified from 60 ml of peripheral blood of OAK patients (n=14, 64.0±10.3 years) using JMS Cell-Aid P-type kit (group J) or Zimmer Biomet APS kit (group Z). Surplus PRPs were frozen at -80°C. Using flow cytometry, the expression of CD62P, a marker for activated platelets, was analyzed in peripheral blood, freshly prepared PRP, and frozen-thawed PRP.
Results
The average CD62P positive rates for platelets in peripheral blood (10.5% in group J and 17.6% in group Z) were not significantly different. The platelet activation rates increased significantly in fresh PRP (15.6% in group J and 26.5% in group Z) and were significantly different between the two groups. The platelet activation rates in frozen-thawed PRP (53.5% in group J and 84.3% in group Z) further increased significantly and were significantly different between the two groups.
Conclusion
Platelets are known to release their contained humoral factors when activated. The content of humoral factors may be different in PRPs with different platelet activation rates, leading to possibly different treatment effects. In the future, we will verify this effect on target cells.