W. Brown (Irvine, US)
UC Irvine Biomedical EngineeringPresenter Of 1 Presentation
P254 - Method of Fibrin Sealant Application does not Affect Cartilage Graft Adhesion
Abstract
Purpose
Fibrin sealant has become the gold standard fixative for cartilage repair. It is commonly used to fixate MACI (matrix-induced autologous chondrocyte implantation) implants and suspend and fixate particulated cartilage grafts. It may be applied via single application for implant fixation or double application to first obtain hemostasis of bleeding subchondral bone. Therefore, the objective of this study was to determine if the method of fibrin application affects the adhesion of cartilage grafts to subchondral bone.
Methods and Materials
Articular cartilage and subchondral bone explants were harvested from juvenile bovine stifles. Cartilage and bone explants were joined together via a single or double application of commercially available fibrin sealants (Tisseel, Baxter; Vistaseal, Johnson & Johnson). For a single application (SA), 10 µL each of fibrinogen and thrombin were used at the interface. For a double application (DA), 5 µL each of fibrinogen and thrombin were applied to the subchondral bone and allowed to clot, followed by repeat application of 5 µL each of fibrinogen and thrombin to adhere cartilage and bone explants. Composites (n=5) were tested in tension (pull-apart) and lap shear.
Results
Histologically, two distinct, non-contiguous layers of fibrin are visible in the DA group, compared to a single layer in the SA group (Fig.1). However, Student’s t-tests showed no significant differences between SA and DA in mechanical properties for either sealant (Fig.2). A post hoc power analysis indicated results were similar between SA and DA groups.
Figure 1:
Figure 2:
Conclusion
No differences in adhesive properties were seen with either fibrin sealant based on application method. Therefore, the number of fibrin applications to fixate cartilage repair implants may not warrant significant attention intraoperatively. However, the adhesive stiffness and strength of both sealants were 1,000x lower than the tensile properties of native cartilage, which motivates the need for a better cartilage implant adhesive.
Presenter Of 1 Presentation
P254 - Method of Fibrin Sealant Application does not Affect Cartilage Graft Adhesion
Abstract
Purpose
Fibrin sealant has become the gold standard fixative for cartilage repair. It is commonly used to fixate MACI (matrix-induced autologous chondrocyte implantation) implants and suspend and fixate particulated cartilage grafts. It may be applied via single application for implant fixation or double application to first obtain hemostasis of bleeding subchondral bone. Therefore, the objective of this study was to determine if the method of fibrin application affects the adhesion of cartilage grafts to subchondral bone.
Methods and Materials
Articular cartilage and subchondral bone explants were harvested from juvenile bovine stifles. Cartilage and bone explants were joined together via a single or double application of commercially available fibrin sealants (Tisseel, Baxter; Vistaseal, Johnson & Johnson). For a single application (SA), 10 µL each of fibrinogen and thrombin were used at the interface. For a double application (DA), 5 µL each of fibrinogen and thrombin were applied to the subchondral bone and allowed to clot, followed by repeat application of 5 µL each of fibrinogen and thrombin to adhere cartilage and bone explants. Composites (n=5) were tested in tension (pull-apart) and lap shear.
Results
Histologically, two distinct, non-contiguous layers of fibrin are visible in the DA group, compared to a single layer in the SA group (Fig.1). However, Student’s t-tests showed no significant differences between SA and DA in mechanical properties for either sealant (Fig.2). A post hoc power analysis indicated results were similar between SA and DA groups.
Figure 1:
Figure 2:
Conclusion
No differences in adhesive properties were seen with either fibrin sealant based on application method. Therefore, the number of fibrin applications to fixate cartilage repair implants may not warrant significant attention intraoperatively. However, the adhesive stiffness and strength of both sealants were 1,000x lower than the tensile properties of native cartilage, which motivates the need for a better cartilage implant adhesive.