R. Nakajima (Tokyo, JP)
Juntendo University Hospital Orthopaedics & Sports MedicinePresenter Of 1 Presentation
P203 - Comparative study of Bioactive substances (BS) in Platelet-Rich Plasma (PRP) and PRP-Freeze-Drying (FD)
Abstract
Purpose
PRP is used for the treatment of sports injuries and arthropathy. PRP-FD is made by activating PRP during the purification process to release BS and processing the liquid component into powder form. However, there are few reports that have examined the amount of BS in PRP and PRP-FD.
Methods and Materials
Blood samples were collected from 10 healthy males (mean 46.5±15 (mean±SD)), and leukocyte-rich PRP (LR-PRP group), leukocyte-poor PRP (LP-PRP group), and PRP-FD (PRP-FD group) were prepared, and blood cell counts (leukocytes, platelets) and BS (TGF-β, EGF, FGF, IL1Ra, IL-6) were measured. LP-PRP and LR-PRP were activated by freezing and thawing before measurement. SPSS (ver. 27.0) was used for statistics, and ANOVA was performed followed by Tukey-Kramer post-hoc test with p<0.05.
Results
The platelet count was 66.8/66.5/49.2 (x104/μL) in the LP-PRP/LR-PRP/PRP-FD group respectively, and the white blood cell count was 1.73/10.9/2.35 (x103/μL) (p<0.001), which was significantly higher in the LR-PRP group. In BS, TGF-β and EGF were significantly higher in the LR-PRP group at 4.76/12.6/6.10(x103 pg/μL)(p<0.05), 15.5/23.8/16.7(x102 pg/μL)(p<0.05), but there were no significant difference between the PRP-FD and LP-PRP groups. FGF-2 was significantly higher in the PRP-FD group at 60.5/37.7/80.8(pg/μL)(p<0.05). IL1Ra was significantly higher in the LR-PRP group at 0.22/14.4/4.59 (x103 pg/μL) (p<0.05), but there was no significant difference between the PRP-FD and LP-PRP groups, and IL-6 was 6.52/5.15/5.35 (pg/μL), with no significant difference between the groups.
Conclusion
PRP-FD can be stored for a long period at room temperature, and has the advantage that it can be used at any medical institution in urgent situations when sports injuries occur. In the present study, it was suggested that PRP-FD has the same anti-inflammatory effect as LP-PRP and is likely to have the same tissue repairing effect as LP-PRP and LR-PRP.
Presenter Of 1 Presentation
P203 - Comparative study of Bioactive substances (BS) in Platelet-Rich Plasma (PRP) and PRP-Freeze-Drying (FD)
Abstract
Purpose
PRP is used for the treatment of sports injuries and arthropathy. PRP-FD is made by activating PRP during the purification process to release BS and processing the liquid component into powder form. However, there are few reports that have examined the amount of BS in PRP and PRP-FD.
Methods and Materials
Blood samples were collected from 10 healthy males (mean 46.5±15 (mean±SD)), and leukocyte-rich PRP (LR-PRP group), leukocyte-poor PRP (LP-PRP group), and PRP-FD (PRP-FD group) were prepared, and blood cell counts (leukocytes, platelets) and BS (TGF-β, EGF, FGF, IL1Ra, IL-6) were measured. LP-PRP and LR-PRP were activated by freezing and thawing before measurement. SPSS (ver. 27.0) was used for statistics, and ANOVA was performed followed by Tukey-Kramer post-hoc test with p<0.05.
Results
The platelet count was 66.8/66.5/49.2 (x104/μL) in the LP-PRP/LR-PRP/PRP-FD group respectively, and the white blood cell count was 1.73/10.9/2.35 (x103/μL) (p<0.001), which was significantly higher in the LR-PRP group. In BS, TGF-β and EGF were significantly higher in the LR-PRP group at 4.76/12.6/6.10(x103 pg/μL)(p<0.05), 15.5/23.8/16.7(x102 pg/μL)(p<0.05), but there were no significant difference between the PRP-FD and LP-PRP groups. FGF-2 was significantly higher in the PRP-FD group at 60.5/37.7/80.8(pg/μL)(p<0.05). IL1Ra was significantly higher in the LR-PRP group at 0.22/14.4/4.59 (x103 pg/μL) (p<0.05), but there was no significant difference between the PRP-FD and LP-PRP groups, and IL-6 was 6.52/5.15/5.35 (pg/μL), with no significant difference between the groups.
Conclusion
PRP-FD can be stored for a long period at room temperature, and has the advantage that it can be used at any medical institution in urgent situations when sports injuries occur. In the present study, it was suggested that PRP-FD has the same anti-inflammatory effect as LP-PRP and is likely to have the same tissue repairing effect as LP-PRP and LR-PRP.