I. Vacková (Praha 4, CZ)
Institute of PhysiologyPresenter Of 1 Presentation
P226 - Clinically Relevant Process of Mesenchymal Stromal Cells Culture and Storage in 3D Scaffolds for Cartilage Regeneration
Abstract
Purpose
Implantation of Wharton’s jelly multipotent mesenchymal stromal cells (WJ-MSCs) can be considered attractive therapeutic option in cartilage regeneration due to their potent paracrine activity. The development of clinical-grade WJ-MSCs grafts requires the selection of preferential 3D scaffold, effective method of scaffold repopulation in vitro as well as adjusted conditions of pre-implantation processing (i.e. optimized short-term storage conditions, ensuring preservation of viable cell graft during safety control and transportation from the laboratory to clinical setting).
The aim of the study was to compare the seeding efficiency, growth and distribution of WJ-MSCs within two commercially available 3D scaffolds, approved for cartilage reconstruction (chondrotissue® and HYALOFAST®) as well as to determine the viability and therapeutic properties of cells after up to 6 days storage at ambient temperatures.
Methods and Materials
Human WJ-MSCs were in vitro seeded into the 3D scaffolds using blood-plasma-based hydrogels. The metabolic activity of cells and cell distribution within the scaffolds were assessed on day 1, 4 and 7 of culture. After 7 days, cell-seeded scaffolds were stored in sealed environment at +25°C for 48 h and 6 days, mimicking the conditions of clinical graft processing. After storage, the viability, metabolic activity and paracrine properties of cells were analysed.
Results
We detected distinct behaviour of WJ-MSCs within two types of 3D scaffolds during 7 days of culture, particularly in cell proliferation/scaffold repopulation capacity. We observed higher cell recovery and preservation of functional properties after storage at ambient temperatures in 3D grafts-based on HYALOFAST® implants. The variability of cell behaviour within the scaffolds was lower in HYALOFAST®. However, the adjustment of cell culture conditions allowed decreasing the variations observed during 3D cell culture within chondrotissue® and resulted in overall improvement of cell recovery after short-term storage.
Conclusion
Here we provide valuable practical insights into generation of 3D WJ-MSCs-based graft for clinical application.
Supported by MH-CR NV-19-06-00355
Presenter Of 1 Presentation
P226 - Clinically Relevant Process of Mesenchymal Stromal Cells Culture and Storage in 3D Scaffolds for Cartilage Regeneration
Abstract
Purpose
Implantation of Wharton’s jelly multipotent mesenchymal stromal cells (WJ-MSCs) can be considered attractive therapeutic option in cartilage regeneration due to their potent paracrine activity. The development of clinical-grade WJ-MSCs grafts requires the selection of preferential 3D scaffold, effective method of scaffold repopulation in vitro as well as adjusted conditions of pre-implantation processing (i.e. optimized short-term storage conditions, ensuring preservation of viable cell graft during safety control and transportation from the laboratory to clinical setting).
The aim of the study was to compare the seeding efficiency, growth and distribution of WJ-MSCs within two commercially available 3D scaffolds, approved for cartilage reconstruction (chondrotissue® and HYALOFAST®) as well as to determine the viability and therapeutic properties of cells after up to 6 days storage at ambient temperatures.
Methods and Materials
Human WJ-MSCs were in vitro seeded into the 3D scaffolds using blood-plasma-based hydrogels. The metabolic activity of cells and cell distribution within the scaffolds were assessed on day 1, 4 and 7 of culture. After 7 days, cell-seeded scaffolds were stored in sealed environment at +25°C for 48 h and 6 days, mimicking the conditions of clinical graft processing. After storage, the viability, metabolic activity and paracrine properties of cells were analysed.
Results
We detected distinct behaviour of WJ-MSCs within two types of 3D scaffolds during 7 days of culture, particularly in cell proliferation/scaffold repopulation capacity. We observed higher cell recovery and preservation of functional properties after storage at ambient temperatures in 3D grafts-based on HYALOFAST® implants. The variability of cell behaviour within the scaffolds was lower in HYALOFAST®. However, the adjustment of cell culture conditions allowed decreasing the variations observed during 3D cell culture within chondrotissue® and resulted in overall improvement of cell recovery after short-term storage.
Conclusion
Here we provide valuable practical insights into generation of 3D WJ-MSCs-based graft for clinical application.
Supported by MH-CR NV-19-06-00355