N. Kadir (Singapore, SG)
National University of Singapore Orthopaedic surgeryPresenter Of 1 Presentation
P223 - Mechano-conditioned Mesenchymal Stem Cells on Electrospun Fibers Enhanced the Paracrine Activities for Cartilage Repair
Abstract
Purpose
The clinical approach of articular cartilage repair remains a challenge due to its suboptimal quality of repair. MSCs are known to secrete a plethora of biologically active factors known as secretome. Secretome profiles of MSCs are reflective of their local microenvironments and able to create an impact on the surrounding cells, eliciting regenerative responses that open up the opportunity of exploiting MSCs towards a cell-free therapy for cartilage repair. The conventional method of culturing MSCs on a tissue culture plate might not have provided the physiological microenvironment for optimum secretome production. This study explored the potential of surface topographies in influencing the MSC secretome production and the possibility of these factors in enhancing the functionality of MSCs and chondrocytes for cartilage repair.
Methods and Materials
MSCs were cultured on TCP, aligned and randomly oriented electrospun fiber sheets to generate serum-free conditioned media (CM). The paracrine activities of fiber-generated CM were compared against TCP-generated CM via functional assays that assessed chondrogenesis, migration, proliferation, inflammatory modulation and cell survival of MSCs and chondrocytes. Involvement of FAK and ERK mechanotransduction pathways in modulating the MSC secretome were investigated by employing specific inhibitors.
Results
We showed that conditioned media of MSCs cultured on electrospun fiber sheets compared to that generated from TCP have improved secretome yield and profile that promote migration, proliferation, MSC chondrogenesis, as well as mitigate inflammation and protect chondrocytes from apoptosis. These functional impact of morphological-dependent MSC secretome generated from electrospun fiber sheets were shown to involve FAK and ERK mechanotransduction pathway.
Conclusion
This study demonstrates the potential of employing electrospun fiber sheets to generate mechano-conditioned MSC secretome with improve paracrine activities for cartilage regeneration.
Presenter Of 1 Presentation
P223 - Mechano-conditioned Mesenchymal Stem Cells on Electrospun Fibers Enhanced the Paracrine Activities for Cartilage Repair
Abstract
Purpose
The clinical approach of articular cartilage repair remains a challenge due to its suboptimal quality of repair. MSCs are known to secrete a plethora of biologically active factors known as secretome. Secretome profiles of MSCs are reflective of their local microenvironments and able to create an impact on the surrounding cells, eliciting regenerative responses that open up the opportunity of exploiting MSCs towards a cell-free therapy for cartilage repair. The conventional method of culturing MSCs on a tissue culture plate might not have provided the physiological microenvironment for optimum secretome production. This study explored the potential of surface topographies in influencing the MSC secretome production and the possibility of these factors in enhancing the functionality of MSCs and chondrocytes for cartilage repair.
Methods and Materials
MSCs were cultured on TCP, aligned and randomly oriented electrospun fiber sheets to generate serum-free conditioned media (CM). The paracrine activities of fiber-generated CM were compared against TCP-generated CM via functional assays that assessed chondrogenesis, migration, proliferation, inflammatory modulation and cell survival of MSCs and chondrocytes. Involvement of FAK and ERK mechanotransduction pathways in modulating the MSC secretome were investigated by employing specific inhibitors.
Results
We showed that conditioned media of MSCs cultured on electrospun fiber sheets compared to that generated from TCP have improved secretome yield and profile that promote migration, proliferation, MSC chondrogenesis, as well as mitigate inflammation and protect chondrocytes from apoptosis. These functional impact of morphological-dependent MSC secretome generated from electrospun fiber sheets were shown to involve FAK and ERK mechanotransduction pathway.
Conclusion
This study demonstrates the potential of employing electrospun fiber sheets to generate mechano-conditioned MSC secretome with improve paracrine activities for cartilage regeneration.