P152 - The Influence of Amniotic Suspension Allografts and Bone Marrow Aspirate Concentrate on Osteoarthritic Chondrocytes
Abstract
Purpose
The purpose of this study was to evaluate the effect of BMAC and ASA on inflammation and cartilage metabolism in a cartilage and synovium co-culture model of osteoarthritis.
Methods and Materials
After institutional IRB approval, 17 patients were enrolled at the time of total knee arthroplasty for donation of cartilage, synovium, and bone marrow aspirate tissue samples. The aspirate was prepared and centrifuged using a standard commercially available BMAC centrifuge system. Four synoviocyte and cartilage co-culture systems were established per patient to accommodate the 2 biologic treatment groups (BMAC and ASA), one control group at 96 hours, and one baseline control cartilage group. Multiplex ELISA was used to measure concentrations of pro-inflammatory mediators’ interleukin – 1b (IL-1b), interleukin – 6 (IL-6), and tumor necrosis factor – alpha (TNF–a) at 96 hours. Additionally, both chondrocyte and synoviocyte RNA were assayed for collagen type I a1 (COL1A1), collagen type II a1 (COL2A1), collagen type III a1 (COL3A1), aggrecan (ACAN), and cartilage oligomeric matrix protein (COMP). All statistical analyses were performed on STATA v16.1. Normality of data was determined by Shapiro-Wilk test, and non-parametric or parametric tests were utilized where appropriate.
Results
There were no significant differences in cytokine concentration between the 96-hr control group and the BMAC co-culture for any cytokine, although IL-6 was trending towards significance (Control: 856.3±346.2 vs BMAC: 662.3±346.8, p=.08). There was a significantly lower concentration of IL-1B in the ASA group compared to the control group (ASA: 11.2±13.9 vs Control: 20.2±39.5, p=.04) (Table 1). No significant differences in expression were observed for Col1A1, Col2A1, Col3A1, COMP or ACAN in either cartilage or synovium samples across the three groups.
Conclusion
The early results from this study suggest that ASA may have anti-inflammatory properties and promote the expression of major extracellular matrix genes in both osteoarthritic chondrocytes and synoviocytes involved in cartilage matrix metabolism.
