Autonomous University of Queretaro
Natural Sciences
My research interests are divided in three lines of research: 1. Development of vaccines and diagnostic methods against ticks and the diseases they transmit. It is based on the study of the genes of ticks and of the pathogens that cause tick-borne diseases in cattle and other species, as well as the potential of the encoded antigens to be used in new vaccines or diagnostic methods against these diseases using genomic, proteomic and bioinformatics techniques, as well as humoral and cellular immunology. 2. Study of the vector-parasite interaction in the transmission of hemoparasites in bovines. The aim is to investigate at the cellular and molecular level, the mechanisms of invasion of bovine hemopathogens that are transmitted by ticks such as Babesia bovis, Babesia bigemina and Anaplasma marginale. The long-term goal is the development of methods that prevent the transmission of these pathogens by ticks to cattle. 3. The use of modern methodologies for the study of disease mechanisms in veterinary medicine. With knowledge of molecular biology, genomics, molecular immunology, and bioinformatics, it is possible to study the mechanisms of invasion, infection and transmission of pathogens to propose improvements in the prevention and diagnosis of diseases of veterinary importance.

Presenter of 2 Presentations

Video On-Demand

THE NEED FOR BETTER TOOLS TO IDENTIFY VACCINE CANDIDATE ANTIGENS FOR TICKS AND TICK-BORNE PATHOGENS (ID 1497)

Session Type
Video On-Demand
Date
08/21/2022
Session Time
18:00 - 21:00
Room
Video On-Demand
Lecture Time
19:10 - 19:30
Onsite or Pre-Recorded
Pre-Recorded
03. Parasites of domestic and wild animals

EFFICACY EVALUATION OF A MULTIEPITOPIC RECOMBINANT PROTEIN AS A VACCINE AGAINST BABESIA BIGEMINA (ID 331)

Session Type
03. Parasites of domestic and wild animals
Date
08/24/2022
Session Time
15:00 - 16:30
Room
Hall B3.M5+6
Lecture Time
15:45 - 15:50
Onsite or Pre-Recorded
Onsite

Abstract

Introduction

Bovine babesiosis is a disease caused by intraerythrocytic parasites of the genus Babesia. This disease is transmitted by ticks, represents a major animal health problem, and has an important economic impact due to decreased production, treatment, and death of infected animals. There is no recombinant vaccine in the world for the prevention of bovine babesiosis. In this study, a multiepitopic recombinant protein, was evaluated as a vaccine antigen against Babesia bigemina.

Methods

Five cattle were immunized with the recombinant protein; three immunizations were performed on days 0, 21, and 42, using 100 μg of the protein in 1 ml of adjuvant for the treatment group, while the control group, also composed of 5 animals, was only immunized with adjuvant. Vaccine efficacy was assessed by challenging all animals with a field strain of B. bigemina at a dose of 1 x 108, parasitized erythrocytes (PE) and measuring the parameters of rectal temperature (RT), packed cell volume (PCV) and percentage of PE (PPE) for 10 days post infection.

Results

The PEP of the vaccinated group was significantly lower (p<0.5) than that of the control group on three of the post-challenge days, and the number of days of parasitemia was lower than that of the control group. Most of the animals in the control group had fever for three days or more and a reduction in VCA of 40% or more, while none of the animals in the vaccinated group reached these conditions. On day 11 post-infection, the animals in the control group were treated while none of the animals in the vaccinated group required treatment.

Conclusions

Cattle immunized with the recombinant multiepitopic vaccine were protected against a controlled challenge with virulent B. bigemina and this antigen can be considered a vaccine candidate against this disease.

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