Presenter of 2 Presentations
THE NEED FOR BETTER TOOLS TO IDENTIFY VACCINE CANDIDATE ANTIGENS FOR TICKS AND TICK-BORNE PATHOGENS (ID 1497)
EFFICACY EVALUATION OF A MULTIEPITOPIC RECOMBINANT PROTEIN AS A VACCINE AGAINST BABESIA BIGEMINA (ID 331)
Abstract
Introduction
Bovine babesiosis is a disease caused by intraerythrocytic parasites of the genus Babesia. This disease is transmitted by ticks, represents a major animal health problem, and has an important economic impact due to decreased production, treatment, and death of infected animals. There is no recombinant vaccine in the world for the prevention of bovine babesiosis. In this study, a multiepitopic recombinant protein, was evaluated as a vaccine antigen against Babesia bigemina.
Methods
Five cattle were immunized with the recombinant protein; three immunizations were performed on days 0, 21, and 42, using 100 μg of the protein in 1 ml of adjuvant for the treatment group, while the control group, also composed of 5 animals, was only immunized with adjuvant. Vaccine efficacy was assessed by challenging all animals with a field strain of B. bigemina at a dose of 1 x 108, parasitized erythrocytes (PE) and measuring the parameters of rectal temperature (RT), packed cell volume (PCV) and percentage of PE (PPE) for 10 days post infection.
Results
The PEP of the vaccinated group was significantly lower (p<0.5) than that of the control group on three of the post-challenge days, and the number of days of parasitemia was lower than that of the control group. Most of the animals in the control group had fever for three days or more and a reduction in VCA of 40% or more, while none of the animals in the vaccinated group reached these conditions. On day 11 post-infection, the animals in the control group were treated while none of the animals in the vaccinated group required treatment.
Conclusions
Cattle immunized with the recombinant multiepitopic vaccine were protected against a controlled challenge with virulent B. bigemina and this antigen can be considered a vaccine candidate against this disease.