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01/01/1970
CHAIR INTRODUCTION (ID 2087)
WELCOME, INTRODUCTION TO ONE HEALTH EJP; TOXOSOURCES: TOXOPLASMA GONDII SOURCES QUANTIFIED (ID 1525)
MEME: MULTI-CENTRE STUDY ON ECHINOCOCCUS MULTILOCULARIS AND ECHINOCOCCUS GRANULOSUS S.L. IN EUROPE: DEVELOPMENT AND HARMONISATION OF DIAGNOSTIC METHODS IN THE FOOD CHAIN (ID 1526)
Abstract
Abstract Body
MEME is an international multicentre collaborative project that aims to fill research gaps highlighted by international agencies for the detection and control of zoonotic parasites Echinococcus multilocularis (Em) and Echinococcus granulosus sensu lato (Eg), causing alveolar echinococcosis (AE) and cystic echinococcosis (CE), respectively.
MEME current achievements:
· Production of Standard Operating Procedures and sampling of different matrices from naturally or experimentally infected definitive and intermediate animal hosts.
· Validation of the parasitological (Segmental Sedimentation and Counting Technique, SSCT) and molecular diagnostic (multiplex-PCRs and MC-RT-PCR assay) procedures to detect Em and Eg in different matrices along the food chain.
· Publications on the development and validation of new tools: a) Comparison of two DNA extraction methods and two PCRs for the detection of Em in stool samples; b) Bayesian Analysis of three methods for diagnosis of CE in sheep; c) Microsatellite investigations of Eg cysts; d) Species detection of Eg by novel probe-based real-time PCRs; e) Validated method based on PCR-RFLP and multiplex PCR assay for the identification of Eg species; f) Identification of Eg G1/G3 by SNPs assays.
· Ongoing multicentre studies for the production of data relevant for epidemiological assessments (contamination of vegetables for human consumption by eggs of Em/Eg; prevalence of Em/Eg in dog faeces).
· Dissemination of project results at different levels (general public, scientific community, experts, health authorities and media).