Sonia Benitez (Spain)

Research Institute from Hospital Sant Pau Biochemistry
Dr. Sonia Benitez. Bachelor in Biochemistry (1996) and PhD in Biochemistry (2002) by Autonomous University from Barcelona. From 1996 in the Cardiovascular Biochemistry Group, Biomedical Research Institute IIB Sant Pau; Hospital de Sant Pau, Barcelona. Senior Investigator Website: http://www.recercasantpau.cat/grup/bioquimica-cardiovascular. The main lines of investigation are the following: Lipids and Lipoproteins. The investigation is focused on alterations in lipids and lipoprotein metabolism in pathologies associated with high cardiovascular risk, such as diabetes mellitus. Particularly, a main area of study is the alteration of the qualitative properties (chemical composition, oxidation, enzymatic activities, size) of LDL and HDL in these diseases. Modified LDL as inductor of atherosclerosis. Among modifications of LDL, the main research interest is the study of electronegative LDL, a modified plasma fraction with atherogenic properties. Receptors and intracellular pathways related to modified LDL-induced inflammation and lipid accumulation in cells, mainly in monocytes and macrophages, are an area of expertise. Atherothrombotic ischemic stroke. The investigation is particularly focused on the role of modified lipoproteins and inflammatory markers in ischemic stroke associated with carotid atherosclerosis. An important aim is the search for biomarkers to assess plaque inflammation and vulnerability in carotid atherosclerosis leading to ischemic stroke.

Author Of 1 Presentation

 Conference Calendar

O044 - Cytotoxic and inflammatory effects induced by secretome from epicardial adipose tissue of diabetic patients in human cardiomyocytes are partly reverted by HDL and apoJ (ID 422)

Session Type
Rapid Fire Session
Session Name
Rapid fire Session - Preclinical (ID 38)
Session Time
14:30 - 15:30
Date
Tue, 01.06.2021
Room
Hall F
Lecture Time
14:48 - 14:53
Presenter

Abstract

Background and Aims

Diabetic patients have high incidence of cardiovascular disease, which is associated with increased volume and functional alterations of epicardial adipose tissue (EAT). We aimed to study the inflammatory and cytotoxic effects induced by EAT from diabetic patients on cardiomyocytes, and the counteracting effect of two cardioprotective molecules: HDL and apolipoprotein J (apoJ).

Methods

EAT was obtained after heart surgery from non-diabetic (ND) and diabetic patients, referred as DM-C or DM according to the presence or not of coronary disease. EAT explants were incubated for 24 h, and the secretomes were added to AC16 human cardiomyocytes in the presence or absence of HDL or apoJ. After 24 hours’ incubation, inflammation was assessed by measuring the release of IL6 and MCP1 by ELISA, cytotoxicity was determined by annexin V staining by flow-cytometry, and the expression of selected genes was evaluated by real-time PCR

Results

Secretomes induced the release of MCP1 and IL6 in AC16, being that from DM the major inductor. Secretome from ND had no cytotoxic effect, whereas secretome from diabetic patients induced two-fold the mortality in AC16. The addition of HDL and apoJ inhibited the secretome-induced inflammatory and cytotoxic effects. Secretome from diabetic patients, mainly from DM-C, induced increased expression of genes related to lipid metabolism: DGAT2, PLIN2, PPARα and PPARγ (1.5-2 fold versus ND).

Conclusions

In summary, secretome from EAT of diabetic patients induced increased inflammatory and cytotoxic response in AC16 cardiomyocytes compared with EAT from ND subjects. Both effects were partially inhibited by HDL and apoJ.

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Presenter of 1 Presentation

 Conference Calendar

O044 - Cytotoxic and inflammatory effects induced by secretome from epicardial adipose tissue of diabetic patients in human cardiomyocytes are partly reverted by HDL and apoJ (ID 422)

Session Type
Rapid Fire Session
Session Name
Rapid fire Session - Preclinical (ID 38)
Session Time
14:30 - 15:30
Date
Tue, 01.06.2021
Room
Hall F
Lecture Time
14:48 - 14:53
Presenter

Abstract

Background and Aims

Diabetic patients have high incidence of cardiovascular disease, which is associated with increased volume and functional alterations of epicardial adipose tissue (EAT). We aimed to study the inflammatory and cytotoxic effects induced by EAT from diabetic patients on cardiomyocytes, and the counteracting effect of two cardioprotective molecules: HDL and apolipoprotein J (apoJ).

Methods

EAT was obtained after heart surgery from non-diabetic (ND) and diabetic patients, referred as DM-C or DM according to the presence or not of coronary disease. EAT explants were incubated for 24 h, and the secretomes were added to AC16 human cardiomyocytes in the presence or absence of HDL or apoJ. After 24 hours’ incubation, inflammation was assessed by measuring the release of IL6 and MCP1 by ELISA, cytotoxicity was determined by annexin V staining by flow-cytometry, and the expression of selected genes was evaluated by real-time PCR

Results

Secretomes induced the release of MCP1 and IL6 in AC16, being that from DM the major inductor. Secretome from ND had no cytotoxic effect, whereas secretome from diabetic patients induced two-fold the mortality in AC16. The addition of HDL and apoJ inhibited the secretome-induced inflammatory and cytotoxic effects. Secretome from diabetic patients, mainly from DM-C, induced increased expression of genes related to lipid metabolism: DGAT2, PLIN2, PPARα and PPARγ (1.5-2 fold versus ND).

Conclusions

In summary, secretome from EAT of diabetic patients induced increased inflammatory and cytotoxic response in AC16 cardiomyocytes compared with EAT from ND subjects. Both effects were partially inhibited by HDL and apoJ.

Hide