IC markers against which inhibitors are currently used/evaluated clinically, have not yet been studied in detail in metastases (M) from patients with primary HR+/HER2- BC. In this study, we examined expression levels of these IC markers in multiple M and primary tumors (P) from primary HR+/HER2- BC patients who enrolled in our postmortem tissue donation program UPTIDER (NCT04531696).
326 samples (23 untreated P and 290 postmortem M samples) from 10 UPTIDER patients with primary HR+/HER2- BC underwent bulk RNA sequencing (Lexogen). Quality control (QC) was set for 500000 assigned reads and gene counts were normalized using variance stabilizing transformation. We selected IC markers being clinically investigated in BC (
324/326 samples passed QC (289 M and 22 P) and the IC expression was stable with increasing ssPMI.
This study sheds light on the expression of IC markers in M from patients with HR+/HER2- primary BC as well as on the impact of histology and ER-expression.
NCT04531696.
Laboratory for Translational Breast Cancer Research.
KU Leuven, Leuven, Belgium.
K. Punie: Financial Interests, Institutional, Advisory Board: AstraZeneca, Novartis, Roche, Vifor Pharma, Eli Lilly, Pierre Fabre, McCann Health, Roularta, Teva, Gilead Sciences, Pfizer, Gilead, MSD; Financial Interests, Institutional, Invited Speaker: Pfizer, Roche, Novartis, Eli Lilly, Mundi Pharma, MSD, Medscape, MSD; Financial Interests, Institutional, Other, Consultancy: Roche; Financial Interests, Personal, Other, Consultancy: Gilead, Novartis, MSD, Roche; Financial Interests, Personal, Invited Speaker: AstraZeneca, Sanofi; Financial Interests, Personal, Advisory Board: Seagen; Financial Interests, Institutional, Funding: Sanofi; Non-Financial Interests, Principal Investigator: EORTC 1745-ETF-BCG trial; Non-Financial Interests, Other, Committee member: ESMO Young Oncologists Committee; Non-Financial Interests, Invited Speaker: BSMO; Non-Financial Interests, Other, Committee Member: ESMO Resilience Task Force; Non-Financial Interests, Advisory Role: Commission Personalized Medecine Federal Government Belgium; Non-Financial Interests, Advisory Role, External Scientific Advisor: European Medicine Agency. H. Wildiers: Financial Interests, Institutional, Advisory Board: Roche, Lilly, AstraZeneca, Daiichi Sankyo, PSI Cro AG, KCE, MSD, MSD, E Squared Communications LLC; Financial Interests, Institutional, Invited Speaker: Eisai, AstraZeneca; Financial Interests, Institutional, Other, Consultancy fee: AbbVie, Immutep Pty; Financial Interests, Institutional, Expert Testimony: Daiichi Sankyo; Financial Interests, Institutional, Other, Consultancy: Daiichi Sankyo; Financial Interests, Institutional, Research Grant, Grant to the Leuven Breast Center to support the research database: Roche; Financial Interests, Institutional, Research Grant, Grant to institute to perform a multicentric national academic trial: Novartis; Other, Travel & accomodations: Pfizer; Other, Travel & accomodation: Roche; Other, Subscription fee: Gilead. G. Floris: Financial Interests, Institutional, Advisory Board: AstraZeneca. All other authors have declared no conflicts of interest.