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O092 - MICROBIAL DIVERSITY IS AFFECTED BY COGNITIVE BEHAVIORAL THERAPY AND MINDFULNESS INTERVENTION AND ACCOMPANIED BY AN INFLAMMATORY RESPONSE IN CROHN'S DISEASE (ID 962)
Abstract
Background and Aims
Crohn's disease (CD) is a chronic inflammatory bowel disease associated with psychological stress and intestinal microbial changes. At certain taxonomic levels, the structure of the gut microbiota is significantly altered in CD patients compared to that of healthy individuals. Here, we determined whether a 3-month period of Cognitive Behavioral and Mindfulness-Based Stress Reduction (COBMINDEX), which improved the wellbeing and inflammatory state of CD patients, may also affect their gut microbiome.
Methods
Gut microbiota, circulating inflammatory markers and hormones were studied and compared among 25 CD patients before (T1) and after (T2) COBMINDEX, and 25 matched CD wait-list controls at the corresponding time-points. Microbiota analysis examined relative abundance, alpha and beta diversity measurements, and correlations with inflammatory parameters.
Results
CD patients exhibited a characteristic microbial profile which constitutes mainly of Proteobacteria (%17.71), Firmicutes (%65.56), Actinobacteria (%8.46) and Bacteroidetes (%6.24). Significant changes in beta diversity (Bray-Curtis dissimilarity index) were observed over time in both CD patient groups with an increase among COBMINDEX (p=0.03), and a decrease among wait-list (p=0.00019). Microbial changes in IBD-associated phyla following COBMINDEX, were accompanied by changes in inflammatory markers. Changes in seven untied-to-IBD phyla significantly correlated with inflammatory markers.
Conclusions
Our results show that microbial diversity is connected to the inflammatory profile of CD patients and is significantly affected by COBMINDEX. Linked changes between phyla and inflammatory markers, demonstrated a microbial-inflammatory relationship among CD patients. Lastly, correlations between changes in abundances of untied to CD taxa, with inflammatory markers, hinted new microbial targets in CD.