Presenter of 2 Presentations
IS010 - COVID-19 AND HASHIMOTO’S DISEASE (ID 800)
Abstract
Abstract Body
COVID-19 and autoimmune thyroiditis (AIT) often are comorbid because of high prevalence of both entities and possible viral induction of autoimmunity. Molecular mimicry of viral and self antigens is one of the key factors in autoimmunity promotion. We studied correlative dynamics of ferritin level and few immunoendocrine parameters in people with AIT who suffered from COVID-19 and explored possible molecular mimicry of SARS-CoV2 and human thyroid antigens. 409 AIT patients were involved, 214 of them suffered from COVID-19 (23 patients were examined during the acute phase of COVID-19, and 191 - 1 to 18 months after negative PCR test achieved). Clinical blood analyses, serum levels of ferritin, TSH, freeT4, freeT3, prolactin, cortisol, and autoantibodies (Aab) towards thyroglobulin, thyroperoxidase and TSH receptors were measured. We also performed a bioinformatic analysis of probable pentapeptide sharing between human thyroid antigens and SARS- CoV-2 spike glycoprotein, based on UNIPROT and NCBI databases with use of Python language. The level of ferritin in individuals with AIT who did not suffer from COVID-19 fluctuated within the normal range with a tendency to its lower limit and inversely correlated with prolactin (r=-0.11). Ferritin level reflected the severity of COVID-19, especially lung damage. Blood lymphocyte absolute count and relative % in acute COVID-19 decreased with subsequent normalization. This can be related to emigration of lymphocytes from the blood to the involved tissues during acute COVID-19 and may play a part in multi-organ postcovid autoimmune disorders. Ferritin strongly positively correlated with Aab to TSH receptors (r=0.82); less strongly – with hemoglobin (r=0.47), and weakly – with body mass index (r=0.23), fT3 (r=0.13), and iron levels (r=0.07). Bioinformatic study revealed 6 pentapeptides common for thyroid autoantigens and SARS-CoV-2 (P0DTC2) spike glycoprotein, shown in table 1 below:
| Thyroid autoantigens | Shared pentapeptides |
| Thyroglobulin (P01266) | FNFSQ, SAIGK, LDSKT |
| Thyrotropin receptor (P16473) | ICGDS, LLPLV |
| Thyroid peroxidase (P07202) | RAAEI |
O066 - SILICONE, MAMMAL GLAND AND AUTOIMMUNITY: A YEAR FOLLOW-UP IN MAMMOPLASTY RECIPIENTS (ID 965)
Abstract
Background and Aims
We performed a prospective follow-up of 119 aesthetic/reconstructive breast surgeries, checking every female before, 3, 6 and 12 months after the operation.
Methods
In 90 cases silicone breast implants (SBI) were used, 29 cases of breast surgery without silicone (BSWS) constituted control group. Blood serum levels of: prolactin, TSH, thyroid hormones, calcitriol, testosterone, estrogens and 9 various autoantibodies were measured. ASIA questionnaire interview was taken before and year after surgery. Local tissue specimens obtained from 12 females during and 6 months after operation were histologically analyzed.
Results
Only in SBI (but not in BSWS) significant increase in anti-TSHR autoantibodies occurred (both regarding seropositive cases number, and average anti-TSHR concentration which exceeded normal range). 18,5% of SBI patients (but no one among BSWS) showed significant gain in anti-MCV and anti-cardiolipin autoantibodies (but within normal ranges). Autoantibodies mentioned positively correlated with each other and negatively – with testosterone. While expecting surgery, stress-related/psychogenic hyperprolactinemia manifested in 50% of cases (both in SBI and BSWS), disappearing in 3 months. After a year number of females positive for ASIA critheria increased (both in SBI and BSWS), perhaps resulting from post-operation immune system hyperstimulation. Bioptates showed perifocal inflammation, silicone-containing microgranulomas and CD3/CD4+ lymphocyte infiltration. Hence, SBI may increase anti-thyroid autoimmunity in healthy recipients, presumably due to its adjuvant effect. Before surgery calcitriol level was low-normal, with significant increase 12 months after SBI, probably reflecting vitamin D activating compensatory response to adjuvant effect.
Conclusions
SBI-related risk of autoimmune thyropathies should be taken into account among contraindications and during dispensary observation.