Tokyo Medical and Dental University
Institute of Biomaterials and Bioengineering
Kohji Mitsubayashi received his Ph.D. from The University of Tokyo in 1994. Since 2003, he has been a Professor at the Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University. His research interests include wearable biosensors, gas sensors for non-invasive bio-monitoring, and novel battery-free artificial organs (pancreas, muscles) with “Organic Engine.” He has proposed a new device category “Cavitas sensors” in human body cavities for real-time biomonitoring such as “Soft contact lens glucose sensors”, “Telemetric mouthguard sensors with a Bluetooth transmitter”, etc. In 1988, he also started to develop several types of gas-phase biosensors (bio-sniffer, sniff-cam). They allow to real-time sensing and imaging of target volatiles in exhaled air, skin gas with good sensitivity, gas-selectivity, and in-sensitivity to humidity. Recently, the novel sniff-cam with a UV-LED ring light has been achieved to real-time imaging of transcutaneous volatiles (ethanol and acetaldehyde) after drinking. He has published more than 240 peer-reviewed research articles in International Journals and 20 books including “Chemical, Gas, and Biosensors for Internet of Things and Related Applications” (first editor) on Elsevier publisher in 2019 (AAP’s 2020 PROSE Award in the Chemistry and Physics category by the Association of American Publisher (AAP).

Presenter of 1 Presentation

GAS-PHASE BIOSENSOR FOR EXHALED ACETONE AS AN EARLY DIAGNOSTIC MARKER FOR DIABETES

Session Type
Virtual Oral Presentations Session
Date
Fri, 29.04.2022
Session Time
16:30 - 18:00
Room
Virtual Hall 1.1
Lecture Time
17:26 - 17:34

Abstract

Background and Aims

Acetone in exhaled breath has the potential to be a biomarker for non-invasive monitoring of the progress of diabetes. From this point of view, an acetone bio-sniffer (gas-phase biosensor) for the assessment of acetone in exhaled breath for early diagnosis of diabetes mellitus was developed and applied to the breath acetone analysis.

Methods

NADH-dependent secondary alcohol dehydrogenase (S-ADH) can reduce acetone to be isopropanol with the oxidation of NADH to NAD+. Therefore, the decreasing of NADH fluorescence intensity with the S-ADH reaction can be utilized to determinate acetone concentration. The acetone bio-sniffer was composed of an NADH fluorescence measurement unit, a flow-cell attached to the optical fiber probe, and an enzyme-immobilized membrane. When bio-sniffer contacted the gaseous acetone, the change of fluorescence intensity caused by S-ADH would be detected by the photo detector.

Results

This acetone bio-sniffer showed high sensitivity to acetone vapor. The dynamic range of the sensor was from 20 to 5300 ppb acetone. Then, we applied the bio-sniffer to measure breath acetone concentration. The mean concentration of breath acetone in all healthy subjects was 750.0 ppb. However, the mean exhaled acetone in diabetic patients was 1207.7 ppb, which was much higher than that in healthy subjects and showed a significant difference.

Conclusions

The breath acetone level for diabetic patients was higher than that of healthy subjects. This finding is worthwhile in the study of breath biomarkers for diabetes mellitus diagnosis. This bio-sniffer provides a new kind of analytical tool for the non-invasive early diagnosis of diabetes mellitus in the near future.

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