Presenter of 1 Presentation
GAS-PHASE BIOSENSOR FOR EXHALED ACETONE AS AN EARLY DIAGNOSTIC MARKER FOR DIABETES
Abstract
Background and Aims
Acetone in exhaled breath has the potential to be a biomarker for non-invasive monitoring of the progress of diabetes. From this point of view, an acetone bio-sniffer (gas-phase biosensor) for the assessment of acetone in exhaled breath for early diagnosis of diabetes mellitus was developed and applied to the breath acetone analysis.
Methods
NADH-dependent secondary alcohol dehydrogenase (S-ADH) can reduce acetone to be isopropanol with the oxidation of NADH to NAD+. Therefore, the decreasing of NADH fluorescence intensity with the S-ADH reaction can be utilized to determinate acetone concentration. The acetone bio-sniffer was composed of an NADH fluorescence measurement unit, a flow-cell attached to the optical fiber probe, and an enzyme-immobilized membrane. When bio-sniffer contacted the gaseous acetone, the change of fluorescence intensity caused by S-ADH would be detected by the photo detector.
Results
This acetone bio-sniffer showed high sensitivity to acetone vapor. The dynamic range of the sensor was from 20 to 5300 ppb acetone. Then, we applied the bio-sniffer to measure breath acetone concentration. The mean concentration of breath acetone in all healthy subjects was 750.0 ppb. However, the mean exhaled acetone in diabetic patients was 1207.7 ppb, which was much higher than that in healthy subjects and showed a significant difference.
Conclusions
The breath acetone level for diabetic patients was higher than that of healthy subjects. This finding is worthwhile in the study of breath biomarkers for diabetes mellitus diagnosis. This bio-sniffer provides a new kind of analytical tool for the non-invasive early diagnosis of diabetes mellitus in the near future.