Syeda Sadia Fatima, Pakistan
Aga Khan University Department of Biological and Biomedical SciencesPresenter of 1 Presentation
SIX MONTHS DIETARY AND LIFESTYLE MODIFICATION REDUCES THE CHEMERIN GENE METHYLATION STATUS IN METABOLICALLY UNHEALTHY YOUNG ADULTS: A PILOT STUDY
Abstract
Background and Aims
Environmental factors/stresses play an imperative part in pathophysiology of Obesity and Type 2 diabetes mellitus (T2DM). This pilot study aimed to assess the effect of life style intervention on the degree of methyaltion of Chemerin, Insulin induced gene 2, Pro-opiomelanocortin genes and advanced glycation end products in metabolic healthy and unhealthy subjects.
Methods
Fifty eight subjects between the ages of 20-38 years were recruited from Aga Khan University and categorized as metabolically healthy (Group A n=28) and metabolically unhealthy (Group B n=30) using NCEP-II criteria. Group B subjects were provided a 6 months intervention where they refrained from consuming all forms of processed food, maintained a daily food/calorie diary and performed physical activity for at least 30 minutes each day of the week. Fasting blood glucose, Insulin, Lipid profile, Serum Chemerin, and AGE levels were measure. Methylation specific PCR was performed for Chemerin, POMC and INSIG-2 at both time intervals.
Results
At baseline, serum AGE was elevated in Group B subjects vs Group A (878.611 ± 20.85Vs. 410.19 ± 36.52ng/ml). High methylation potential was seen for genes regulating insulin release and satiety i.e. INSIG 2, POMC while high degree of nonmethylation was seen in Chemerin in Group B compared to Group A. At 6th month reduction in AGE levels (756 ± 14.55ng/ml) along with 3% reduction in degree of Chemerin nonmethylation was observed.
Conclusions
Preliminary data suggests that these changes can be modified with dietary intervention and life style modification.