Rani Sauriasari, Indonesia
Universitas Indonesia Faculty of PharmacyPresenter of 1 Presentation
'ASSOCIATION BETWEEN HYDROGEN PEROXIDE, 8-ISOPGF2Α, AND OXLDL-Β2GP1 COMPLEXES WITH RENAL FUNCTION DECLINE IN TYPE 2 DIABETES MELLITUS PATIENTS
Abstract
Background and Aims
Even though diabetes patients exhibit an increase in oxidative stress, its correlation with declining renal function is not fully understood. The purpose of this study was to determine whether hydrogen peroxide, 8-isoPGF2α, and oxLDL-β2GP1 complexes correlated with renal function decline as shown by eGFR in type 2 diabetes mellitus (T2DM) patients.
Methods
The design of this study was cross-sectional. A total of 227 T2DM patients (134 normoalbuminuria patients and 93 albuminuria patients) were divided based on the eGFR value. Group 1 was patients with eGFR > 60 mL/min/1.73 m2 (n=195) and group 2 was patients with eGFR ≤ 60 mL/min/1.73 m2 (n=32). T2DM patients’ serum and urine specimens were analyzed to measure their serum creatinine and eGFR based on CKD-EPI equation. Serum oxLDL-β2GPI complexes and urine 8-isoPGF2a were measured by ELISA and urine hydrogen peroxide was measured by Ferrous Ion Oxidation Xylenol Orange 1 (FOX-1).
Results
The results showed that oxLDL/β2GPI was higher in group 2 (0.60±0.08 units/mL) than group 1 (0.59±0.04 units/mL) significantly (p=0.04). In contrary, hydrogen peroxide and 8-iso-PGF2α were lower in group 2 than group 1), but not statistically significant. However, no significant correlation between eGFR with oxLDL/β2GPI and the other two parameters. Only oxLDL/β2GPI and hydrogen peroxide showed a significant correlation (r=0.145, p=0.029).
Conclusions
In conclusion, serum oxLDL-β2GP1 has a role in the kidney function decline in T2DM patients that contribute to nephropathy diabetes and showed correlation with hydrogen peroxide, but not for 8-iso-PGF2α.