Mohammad Shabani, Iran
School of Medicine, Iran University of Medical Sciences (IUMS) Biochemistry DepartmentPresenter of 1 Presentation
THE EFFECTS OF NITRIC OXIDE (NO) ON THE IMMUNE SYSTEM USING STZ-INDUCED DIABETIC RAT’S DURING WOUND HEALING
Abstract
Background and Aims
Diabetic wounds are an important area of medical challenges. Wound healing is a dynamic and complex immuno-biochemical process. Impaired wound healing is a common occurrence among diabetics and patients receiving glucocorticoid therapy. Nitric Oxide is a free radical human factor with diverse biological roles. The role of NO in both normal and chronic wound is not clear; this study was designed at gaining further insights into the role of NO in wound healing.
Methods
In this research 14 Sprague Dawley (SD) male rats were assigned into two treatment and control groups. Rats in the diabetic group (n=8) were injected with STZ (65mg/kg i.p.) 9 days before wounding. In the second study steroid-treated rats (n=6) were given cortisone acetate (CA: 10 mg i.m.). Rats received full-thickness dermal wounds. The wound area was measured by the VIA technique. Urine samples were collected 24-hrs before and post-wounding. Urine NO was measured by NO Analyzer. Student-t-test was used for statistical analysis.
Results
Post wound increase in urinary NO3- output was significantly reduced in both diabetic (47%) and CA-treated (40%) rats compared to the control group (136%, P≤ 0.0001). Furthermore, NO3- output in diabetic and CA-treated rats fell within pre-wound baseline levels by day 13, while that of normal controls remained elevated (54%) for the entire 30-day post-wound period.
Conclusions
These results suggest that impaired wound lack normal levels of endogenous NO may be due to reduced activity of immune cells such as macrophages during wound healing as shown by injection of LPS and increased level of NO.