MC4R gene encodes melanocortin receptor type 4. The association of rs17782313 polymorphism MC4R gene with the obesity is proved.
The aim of study is to analyze the changes in body fat mass due to the rs17782313 T/C MC4R gene in overweight patients after 3 and 12 months of diet.
The study involved 16 overweight patients (the mean BMI 34.08±5.59 kg/m2) from 23 to 60 y.o. (the mean age 47.5±11.78). All patients took a balanced diet with the exclusion of simple and the limiting of complex carbohydrates and fats. The changes in body composition were estimated by the bioelectrical impedance analysis. Buccal cells PCR-based genotyping was performed on CFX96 amplifier.
The distribution of rs17782313 polymorphism genotypes corresponded to the Hardy-Weinberg equilibrium (χ2 = 0.17; p = 0.68). The frequency of occurrence of the C allele differed from the European population (9% 24%; 1000 Genomes Project), which can be explained by the small sample size. TT homozygotes showed a significant decrease in body fat mass after 3 months of diet (p= 0.007) compared with C allele carriers (-4.56 ± 2.55 kg vs. + 0.38 ± 1.66 kg). There were no significant differences in changes in body composition in TT homozygotes compared with C-allele carriers after 12 months of diet (p> 0.05).
The presence of the C allele in the homozygous state of the rs17782313 MC4R is associated with less decreasing of fat mass during the 3 months of a generally accepted diet, no further differences in changes in body composition are observed.