The search for new effective ways to treat diabetes is one of the most important problems of medicine. The purpose of study was to determine the levels of lipid peroxidation, oxidative modification of proteins, the content of nitric oxide, Schiff bases and the state of the thiol-disulfide system in the mitochondrial fraction and the total homogenate of the tissue of the liver of the white rat in alloxan diabetes and treatment with taurine.
The study was conducted on outbred white rats. Alloxan diabetes is caused by the administration of 40 mg / kg of alloxan. Lipid peroxidation was determined by the method of Barkovsky EV. Oxidative modification of proteins was determined by the method of Dubinin et al. The amount of protein was determined according to Lowry. Glutathione peroxidase activity was determined by the reduction of hydrogen peroxide and lipid hydroperoxide in the presence of GSH. The optical density of the Schiff bases was determined at a wavelength of 400 nm.
Studies have shown that alloxan diabetes is characterized by the activation of free radical oxidation processes, as well as a decrease in glutathione content and a decrease in the activity of glutathione-containing enzymes. Introduction of taurine leads to a decrease in the content of lipid peroxidation products, partial normalization of the oxidative modification of proteins and Schiff bases in the common liver homogenate of rats with alloxan diabetes.
Thus, we can conclude that the administration of taurine to rats with alloxan diabetes leads to the normalization of oxidative processes.