King George's Medical University
Centre for Advanced Research (CFAR), Faculty of Medicine,
Dr. Shailendra Saxena is faculty of King George's Medical University, Lucknow, India. His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various prestigious international societies/academies including the Royal Society of Medicine, London; Royal College of Pathologists, UK; Royal Societies of Biology and Chemistry, London, United Kingdom; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in the vaccination for Japanese encephalitis by IPIC (UK).

Moderator of 1 Session

Session Type
Oral Presentations
Date
Thu, 24.02.2022
Session Time
10:00 AM - 11:00 AM
Room
Sala A
Session Icon
Pre-Recorded with Live Q&A

Presenter of 1 Presentation

MOLECULAR MECHANISM OF INNATE IMMUNE RESPONSE DURING JAPANESE ENCEPHALITIS VIRUS INFECTION

Session Type
Oral Presentations
Date
Thu, 24.02.2022
Session Time
10:00 AM - 11:00 AM
Room
Sala A
Session Icon
Pre-Recorded with Live Q&A
Lecture Time
10:10 AM - 10:20 AM

Abstract

Background

Japanese encephalitis (JE) is an arboviral disease which accounts for significant pediatric mortality annually in Asia and Australia, including India. Encephalitis is merely the result of viral invasion in CNS and pathological consequences. Neuronal loss is the outcome of both JEV infection of neurons and bystander damage caused by activated microglia. Recent studies have shown that micro RNAs (miRs) holds a major platform during JEV infection and modulate cellular pathways to determine the pathological condition.

Aims

Therefore, the present study was planned to characterize the NS3 gene of JEV GP05 and investigate the role of miRs in controlling the IL-10 expression (Th2 response) during JEV infection in macrophages.

Methods

See Methods in following Figure 1

methods.jpg

Results

We report that miR-98, miR-27a and miR-106b might have a possible role in posttranscriptional regulation of IL-10 gene expression during JEV infection. Our analysis predicted the novel miRs having potential to bind with the 3’UTR region of IL-10 mRNA. From the predicted miRs, miR-98 and miR-27a were selected on the basis of published article showing their role in regulation of IL-10 expression. Novel microRNA miR-106b was selected on the basis of free energy and prediction from the software. Gel PCR, real time PCR (RT-PCR) and western blotting was performed to validate the data. Our results exhibited the correlation between the expression of IL-10 and three miRNAs mainly miR-98, miR-27a miR-106b during JEV infection.

Conclusions

Collectively our data suggested that miR-98, miR-27a and miR-106b might have potential role in posttranscriptional regulation of Interleukin-10 expression during Japanese encephalitis virus infection.

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