Chulalongkorn University
Faculty of Medicine
Director of Excellence Center for Critical Care Nephrology, King Chulalongkorn Memorial Hospital, & Division of Nephrology, Department of Medicine, Chulalongkorn University, Bangkok, Thailand Main research focused in sepsis, tropical infection such as dengue infection, leptospirosis and acute kidney injury. Currently, he has published more than 100 articles in International Peer Review Journal including Lancet, Critical Care Medicine, Critical Care, Kidney International, BMC Medicine, Clinical Journal American Society of Nephrology. Associate Editor in Nephrology Journal , the official Journal of Asian Pacific Society of Nephrology and Subject Editor in BMC Nephrology

Presenter Of 2 Presentations

Dengue & COVID-19: Double Trouble

Session Type
Society Symposium
Date
Wed, 23.02.2022
Session Time
09:45 AM - 10:45 AM
Room
Sala A
Lecture Time
10:00 AM - 10:15 AM

DISCOVERY AND VALIDATION OF CIRCULATING MIRNAS FOR THE CLINICAL PROGNOSIS OF SEVERE DENGUE

Session Type
Oral Presentations
Date
Wed, 23.02.2022
Session Time
09:45 AM - 10:45 AM
Room
Sala E
Lecture Time
09:45 AM - 09:55 AM

Abstract

Background

Dengue is a potentially life-threatening viral infection. Early prognostic markers of severe complications may improve case management and reduce dengue-related mortalities.

Aims

This study aimed to identify circulating microRNAs (miRNAs) as biomarkers for predicting severe dengue.

Methods

Serum samples from dengue-infected patients were collected on the first day of admission. Patients were followed up to 14 days after admission to find out the final diagnosis. The participants were divided into non-severe and severe dengue, as defined by WHO 2009 criteria. Circulating microtranscriptome analysis was performed using the NanoString miRNA Expression Assay. The expression level of candidate miRNAs was then validated by quantitative reverse transcription-PCR (qRT-PCR) method.

Results

The discovery cohort (N=19) identified 37 differentially expressed miRNAs between the two groups. Among these, miR122-5p, miR1246, miR1303, miR574-5p, miR30d-5p, and miR424-5p were selected and further validated in the larger cohort (N=135). The qRT-PCR analysis confirmed that six selected miRNAs expression levels were significantly higher in the severe dengue group than in the non-severe group. Based on receiver operating characteristic (ROC) analysis, miR574-5p and miR1246 displayed the highest diagnostic performance in discriminating severe dengue from non-severe (area under the ROC curve =0.83). Additionally, miR574-5p and miR1246 had high sensitivity and high negative predictive value for detecting severe dengue. Multivariate analysis suggested that serum miR574-5p was an independent predictor of severe dengue (odds ratio 3.30, 95% CI 1.81-6.04; p<0.001).

Conclusions

Together, our study indicated that circulating miRNAs, especially miR574-5p and miR1246, could be promising diagnostic and prognostic biomarkers for severe dengue.

Hide