Adam D. Izsak (Hungary)

semmelweis university Department of Genetics, Cell- & Immunobiology
My name is Adam Izsak. I was born in Israel and currently, I live in Budapest Hungary where I am about to finish my medical academic studies. before I started my studies, I was an officer in the military air force. I wanted to do better for humankind and I knew that I wanted to be a doctor and to save lives so I chose to learn medicine. I come from a Hungarian family and I visited Hungary all throughout my childhood and I loved Budapest so I knew I wanted to live here for a while. During my 6 years as a medical student, I was always drawn to anatomy and surgery and I have participated in competitions in school and abroad in Russia on this fields and threw time and with help of a great teacher I was also exposed to the field of research which I felt fascinated with, my presentation will be on the research that I am currently concurring.

Author Of 1 Presentation

 Conference Calendar

3D CULTIVATION OF PANCREAS CARCINOMA CELL LINES FOR DRUG SCREENING

Session Name
RESEARCH AND INNOVATION (ID 88)
Date
05.07.2021, Monday
Session Time
02:00 PM - 03:00 PM
Room
On-Demand 1 Slide 5 Mins
Presenter
Lecture Time
02:00 PM - 02:05 PM
Session Icon
On Demand

Abstract

Abstract Body

Cells develop in a 3D environment having interactions with ECM and other cells. 3D culturing facilitates differentiation and organization, using micro-structures mimicking in vivo interactions. 3D culturing is used in medical fields, from drug screenings to printing implants.

Aims: (1) to create 3D pancreatic tumor spheroids by magnetic bioprinting and characterize their morphology. (2) to test their behavior after drug treatment and determining IC50 values (3) comer the results of 2D and 3D cultures.

Methods: Two pancreatic adenocarcinoma cell lines, PANC1 and MIAPaCa2, were printed by magnetic bioprinting into 3D spheroids. The spheroid morphology was evaluated in Celldiscoverer7 (Zeiss), while viability determined by Cell Titer Glo assay and xCELLigence system. We tested 6 conventional chemotherapy compounds (e.g., tamoxifen, TIC10, and LCL161) and their novel derivatives.

Results: Both cell lines formed spheroids. Gemcitabine, the widely used anti-tumor agent in pancreas tumor management, showed decreased viability. SMAC mimetic had a spectrum of IC50 values, for LCL161 and another derivative tested of the IC50 was less than 1.85 µM. TIC10 and its derivative and the TMX derivative had very influential results as well. TMX showed the biggest difference between the two culturing techniques; it did not influence the 3D cultures while affecting the 2D cultures.

Conclusion: The 3D culture established by magnetic bioprinting mimics the in vivo cell conditions. the drug treatments on both culturing techniques show similarities in influence, but the degree was different. The novel derivatives tested showed a remarked influence and should be further researched.

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Presenter of 1 Presentation

 Conference Calendar

3D CULTIVATION OF PANCREAS CARCINOMA CELL LINES FOR DRUG SCREENING

Session Name
RESEARCH AND INNOVATION (ID 88)
Date
05.07.2021, Monday
Session Time
02:00 PM - 03:00 PM
Room
On-Demand 1 Slide 5 Mins
Presenter
Lecture Time
02:00 PM - 02:05 PM
Session Icon
On Demand

Abstract

Abstract Body

Cells develop in a 3D environment having interactions with ECM and other cells. 3D culturing facilitates differentiation and organization, using micro-structures mimicking in vivo interactions. 3D culturing is used in medical fields, from drug screenings to printing implants.

Aims: (1) to create 3D pancreatic tumor spheroids by magnetic bioprinting and characterize their morphology. (2) to test their behavior after drug treatment and determining IC50 values (3) comer the results of 2D and 3D cultures.

Methods: Two pancreatic adenocarcinoma cell lines, PANC1 and MIAPaCa2, were printed by magnetic bioprinting into 3D spheroids. The spheroid morphology was evaluated in Celldiscoverer7 (Zeiss), while viability determined by Cell Titer Glo assay and xCELLigence system. We tested 6 conventional chemotherapy compounds (e.g., tamoxifen, TIC10, and LCL161) and their novel derivatives.

Results: Both cell lines formed spheroids. Gemcitabine, the widely used anti-tumor agent in pancreas tumor management, showed decreased viability. SMAC mimetic had a spectrum of IC50 values, for LCL161 and another derivative tested of the IC50 was less than 1.85 µM. TIC10 and its derivative and the TMX derivative had very influential results as well. TMX showed the biggest difference between the two culturing techniques; it did not influence the 3D cultures while affecting the 2D cultures.

Conclusion: The 3D culture established by magnetic bioprinting mimics the in vivo cell conditions. the drug treatments on both culturing techniques show similarities in influence, but the degree was different. The novel derivatives tested showed a remarked influence and should be further researched.

Hide