ePoster Display session ePoster

13P - The role of long non-coding RNA RAMS11 in promoting colorectal cell development and metastasis (ID 294)

Presentation Number
13P
Lecture Time
09:21 - 09:21
Speakers
  • Md Zahirul Islam Khan (Kowloon, Hong Kong PRC)
Session Name
ePoster Display session
Room
ePoster gallery
Date
Tue, 02.03.2021
Time
08:00 - 20:00
Authors
  • Md Zahirul Islam Khan (Kowloon, Hong Kong PRC)
  • Helen K. Law (Kowloon, Hong Kong PRC)

Abstract

Background

Over the past decades, accumulating research evidences revealed that abnormal expressions of long non-coding RNAs (lncRNAs) are associated with tumour initiation, progression, metastasis, and resistance to cancer therapies. Therefore, lncRNAs are considered to be potential biomarkers for many cancer types. In the current study, we examined the expression and molecular mechanisms of a newly identified lncRNA called RNA associated with metastasis 11 (RAMS11) and its association with the development of colorectal cancer (CRC).

Methods

Quantitative RT-PCR was used to determine the expression of RAMS11 in 4 CRC cell lines (DLD-1, HT-29, HCT-116, and SW480) and normal colon cells CCD-112-CoN. To evaluate the biological and physiological functions of RAMS11 in CRC cells, CCK-8 cell proliferation assay, colony formation assay, and wound healing migration assay were performed after RAMS11 knockdown. The expressions of autophagy/apoptosis/mTOR/EMT pathway proteins were determined by Western blotting to evaluate the molecular mechanisms of RAMS11 in CRC cells.

Results

We found that RAMS11 was significantly upregulated in CRC cell lines compared to the normal cells. The knockdown of RAMS11 reduced CRC cells proliferation, and migration through mTOR dependent induction of autophagy, promotion of apoptosis, and inhibition of epithelial-mesenchymal transition (EMT) process.

Conclusions

Overall, our results suggested that RAMS11 is an important oncogenic regulator of CRC initiation and progression whereas, targeting RAMS11 and the related molecular pathways could be used as potential therapeutic strategies for CRC management.

Legal entity responsible for the study

The authors.

Funding

This project is partially supported by: (1) Research grant to HKL including Departmental Seeding Fund and Internal Institutional Research Fund (P0031318-UAHS); (2) Postgraduate studentship from The Hong Kong Polytechnic University for ZIK.

Disclosure

All authors have declared no conflicts of interest.

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