Bladder cancer is a heterogeneous malignancy with wide scale of clinical manifestation. Different chromosomal aberrations have been already identified in bladder tumors. Older age, Asian ancestry and a positive family history of bladder cancer have long been recognized as important risk factors. The aim of the present investigation was to study the major chromosomal aberrations (CA) like deletion, translocation, inversion and mosaic in bladder cancer patients of Tamil Nadu, Southern India.
A total of 62 blood samples were collected from various hospitals in Tamil Nadu, Southern India. Equal numbers of normal healthy subjects were chosen after signing a consent form. Volunteers provided blood samples (5 ml) to establish leukocyte cultures. Cytogenetic studies were performed by using Giemsa-banding technique and finally the results were ensured by using fluorescence in situ hybridization (FISH). Fluorescence in situ hybridization (FISH) has been used to identify the target genes for some of these chromosomal alterations.
Showed frequent CA in chromosomes 1, 8, 9, 10, 11, 13, and 14. By conventional cytogenetics, predominant changes included gain of chromosome 8, loss of Y, deletions of 9q and l0q, and the appearance of double minutes. In the present investigation, major CA like deletion, translocation, inversion and mosaic were identified in experimental subjects. In comparison with experimental subjects, the control subjects exhibited very low levels of major CA (P < 0.05).
In the present study, the high frequency of centromeric rearrangements indicates a potential role for mitotic irregularities associated with the centromere in bladder cancer tumorigenesis. Identification of chromosome alterations may be helpful in understanding the molecular basis of the disease in better manner.
Annamalai University, Tamil Nadu, India
Has not received any funding
All authors have declared no conflicts of interest.