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99P - Effective model for antitumor drugs screening based on 3D growth system of MCF-7

Presentation Number
99P
Lecture Time
17:10 - 17:10
Speakers
  • Tetiana V. Nikolaienko (Kiev, UA)
Session Name
Location
Foyer La Scene, Paris Marriott Rive Gauche, Paris, France
Date
05.03.2018
Time
17:10 - 18:00
Authors
  • Tetiana V. Nikolaienko (Kiev, UA)
  • L. V. Garmanchuk (Kiev, UA)
  • Yu. A. Stupak (Kiev, UA)

Abstract

Background

Gene expression profiles in spheroid cultivated cells are more similar to natural tumors, than profiles of the same cells in monolayer culture. Tumor spheroids are heterogeneous cellular aggregates that, when greater than 500 μm diameter, are frequently characterized by hypoxic regions and necrotic centers. Architecture of three-dimensionally (3D) propagated cells is very similar to avascular tumor areas. The gradient of diffusion in cell aggregates leads to reduced proliferation rates and increased drug resistance. The purpose of the work was to conduct a comparative study between 3D and monolayer growth systems of MCF-7 cells, and prove the value of spheroid model.

Methods

As experimental model was used adhesion line of breast adenocarcinoma MCF-7. Cells in 2D and 3D culture were incubated during 5 days under conditions of starvation. The number of live cells was evaluated using MTT-colorimetric assay. Apoptotic index was assessed by flow cytometry.

Results

MCF-7 cells growth parameters differ significantly in 2D and 3D growth systems. Cells in 2D system are more sensitive to serum starvation then 3D cultures. Cell viability increases dramatically in 3D system. The level of apoptotic and necrotic cells for 2D growth in serum starvation conditions (39.2±7.3% and 33.5±2.8% respectively) were twice increased in comparison with conditions of complete culture medium (19.0±1.3% and 11.4±1.7% respectively), whereas incomplete medium have no detectible effects on 3D cultured cells. However, the 3D cells percentage in G0/G1 phase of the cell cycle was increased in 1.6 times in serum free conditions, whereas it was not changed in complete medium that can indicate similarity to natural tumors.

Conclusions

Therefore, the 3D growth system has been proposed as an adequate and valuable model to study tumor growth and response to therapeutic substances.

Legal entity responsible for the study

Taras Shevchenko National University of Kyiv, ESC “Institute of Biology and Medicine”

Funding

Has not received any funding

Disclosure

All authors have declared no conflicts of interest.

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