Assiut University

Author Of 1 Presentation

Microbiome Poster Presentation

P0680 - The role of gut microbiota in the Egyptian relapsing remitting multiple sclerosis patients. (ID 1599)

Speakers
Presentation Number
P0680
Presentation Topic
Microbiome

Abstract

Background

The gut microbiota assumed to play an essential role in the pathogenesis of MS. It is thought to be involved in modulating the host’s immune system, modifies the integrity and function of the blood brain barrier, triggers autoimmune response, and interacts directly with different cell types present in the CNS, which lead to demyelination at the end. MS has several clinical variants, among which the most frequent is relapsing–remitting MS (RRMS).

Objectives

to assess the role of gut microbiota among the Egyptian RRMS patients and to discover the abundance and diversity of gut microbiota in the patients and healthy age- and sex- matched control group.

Methods

In our cross-sectional study, 40 cases of RR-MS patients (diagnosed according to the McDonald criteria 2017), were consecutively recruited from inpatients ward at the department of Neurology, Assiut University Hospitals, Assiut, Egypt. Together with 30 age and sex matched healthy control subjects. Detailed history, thorough neurologic examinations, MRI brain and whole spine with contrast, CSF analysis, Evoked potentials, complete Laboratory investigations and Expanded Disability Status Scale (EDSS) were carried out for each patient. Stool sample processing and DNA extraction, concentration and copy number of bacterial organisms were estimated for Patients and control groups.

Results

The mean age of the patients was 31.4 ± 8.8, 75% of them were women. The mean disease duration was 29.90 ± 25.79 months. The mean EDSS score was 3.43 ±1.35. There were significant increased Copy number of Desulfovibrio, Actinobacteria, Firmcutes, and Lactic acid bacteria in the patients with RRMS in comparison with control group, in the contrary a significant lower level of Copy number of Clostridium cluster IV group in RR-MS in comparison with control group were found. The other species of gut microbiota showed no significant differences between groups. Patients who had EDSS 3.5 had significant higher copy number of Actinobacteria, Bacteroidetes, and Bifidubacterium, in comparison with patients who had EDSS > 3.5. A significant correlation between EDSS scoring and copy number of Bifidubacterium only with P= 0.04. There was a significant negative correlation between duration of illness and copy number of three species; Firmcutes, Akkermansia, and Lactic acid bacteria (r= - 0.42; P = 0.01, r= - 0.33; P = 0.04, r= - 0.64; P = 0.004 respectively)

Conclusions

The present study demonstrated that the Egyptian RRMS patients had a distinct fecal microbiome compared to healthy controls, with specific changes in certain gut microbes richness among RRMS patients compared to controls.

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