Biomarkers and Bioinformatics Poster Presentation

P0109 - microRNA expression and its association with disability and brain atrophy (ID 1255)

  • I. Casanova
  • C. Lopez De Silanes
  • Y. Aladro Benito
  • L. De Torres
  • I. Casanova
  • S. Abellán Ayuso
  • S. Pérez-Pérez
  • M. García-Martínez
  • M. Eimil Ortiz
  • B. Oyanguren Rodeño
  • M. Gil Moreno
  • R. Terrero Carpio
  • M. Saiz Sepúlveda
  • B. Diaz Montoya
  • E. De Antonio Sanz
  • M. González Salaices
  • M. Domínguez-Mozo
  • R. Alvarez-Lafuente
Presentation Number
Presentation Topic
Biomarkers and Bioinformatics



MicroRNAs (miRNAs) are endogenous, non coding, small RNAs with post-transcriptional regulating functions. They participate in several cellular processes, including inflammation, neurodegeneration and remyelination


To correlate serum miRNAs profile expression with disability, cognitive functioning and brain volume in patients with remitting-relapsing multiple sclerosis (RRMS)


Cross-sectional study in RRMS patients on stable treatment with glatiramer acetate (GA) during at least 6 months.

We selected the 20 best miRNAs candidates for RRMS and cognitive dysfunction through simple topological analysis (Anaxomics ®). MiRNAs profile was determined with LNA-based PCR (Exiqon). Clinical variables were Expanded Disability Status Scale (EDSS), Symbol Digit Modalities Test (SDMT) and brain volume (whole brain volume, grey matter volume, white matter volume, cerebellum volume, basal ganglia volume and T1 lesion load volume) (automatic software NeuroQuant ®). Correlation was analyzed with Spearman correlation coefficient (r) (p<0,05; software: SPSS)


We included 20 patients (13 women), age 38,2 (29,4, 47,8) years, duration of disease: 5,1 (1,5, 8,5) years, and time on GA 2,1 (0,8, 6) years. We found a pathogenic association between miR.146a.5p and has.mir.9.5p with EDSS (r:0,434, p=0,03; r:0,516, p=0,028); miR.146a.5p with SDMT (r:-0,476, p=0,016); has.mir.9.5p with thalamus (r:-0,545, p=0,036), and miR.200c.3p with pallidum and cerebellum (r:-0,675, p=0,002; r:-0,472, p=0,048).


MiRNAs could be useful biomarkers in multiple sclerosis. We would like to highlight the association of proinflammatory has.mir.9.5p with EDSS and thalamus volume. They are needed more studies to confirm this findings.