AN INFLUENCE OF ANTI-THYROPEROXIDASE ANTIBODIES ON THE EXPRESSION OF MCL 1 IN MURINE PLACENTAS

Presenter
  • Elena Borodina (Russian Federation)
Lecture Time
08:10 - 08:16

Abstract

Background and Aims

Presence of antithyroid antibodies is an independent risk factor for pregnancy complications. In this regard, the revealing mechanisms of the antithyroid antibodies influence on placental development is a relevant task.

The aim of the study was to assess the expression of MCL1, which is an anti-apoptotic factor, in the murine placentas after administration of anti-thyroperoxidase antibodies.

Methods

IgG from patients with AIT were injected in mice on the 4th pregnancy day. The titer of antibodies to thyroperoxidase exceeded 1000 U/ml while anti-thyroglobulin and anti-phospholipid antibodies were excluded in these samples. The control group received commercial human IgG. Pregnancy outcomes were evaluated. Immunohistochemical analysis of the MCL1 expression in the placentas was performed.

Results

In the group of mice that received anti-thyroperoxidase antibodies were obtained 70 viable fetuses. In the control group – 195 viable fetuses (p<0.05). The average weight of fetuses and placentas in the studied group was 351 mg and 112 mg, while in the control group - 503 mg and 125 mg, respectively (p<0.05). The IHC assessment revealed a significant decrease in the MCL1 expression in placentas of mice that received anti-TPO antibodies 29.24±1.06% vs 38.57±0.79% in control group (p<0.01).

Conclusions

The decrease in the number of viable fetuses and in the weight of fetuses and placentas after the administration of anti-thyroperoxidase antibodies may be related with the activation of apoptosis in the murine placentas. This might be due to insufficient influence of anti-apoptosis factors, which confirmed by the decrease in the MCL1 expression.

The study supported by a grant 14.W03.31.0009

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