Hakka Naziat, Bangladesh

Child Health Research Foundation Microbiology

Author Of 3 Presentations

NASOPHARYNGEAL CARRIAGE OF STREPTOCOCCUS PNEUMONIAE SEROTYPES AMONG HEALTHY CHILDREN IN NORTH INDIA (ID 318)

Abstract

Background

Streptococcus pneumonia (SP) causes morbidity and mortality among children worldwide. India introduced 13-valent pneumococcal conjugate vaccine (PCV-13) in 2017. Current study was conducted to isolate SP from nasopharyngeal (NP) swabs of healthy children and assess changes in serotypes among PCV vaccinated and unvaccinated children.

Methods

Cross-sectional study was conducted (July-August 2019) in Lucknow District, North India. Children (2-59 months) who had no clear illness/hospitalization (last one-month) were recruited from vaccination-clinics of hospitals. After NP specimen collection, bacterial culture was done using 5% sheep agar-blood plate containing gentamicin. Pneumococcal isolates were identified by optochin-sensitivity and bile-solubility. Serotyping was done using Quellung Method

Results

Of 300 children, 56.7%(170/300) were males and 52.0%(156/300) were 2-11 months. Overall SP colonization rate was 37.6% (113/300). Vaccine serotypes isolated were 18C,19A,19F,23F,3,4,6A,6B,9V. Among 60% (181/300) PCV vaccinated, SP positivity was 37.5% (68/181) and vaccine serotype were 39.7% (27/68). Among 40% (119/300) non-PCV vaccinated-children, 37.8%(47/119)had NP colonization positive and PCV-13 serotypes were 37.7%(17/47). Non-Vaccine serotypes were 10A,15A,15B,15C,21,34,35B,22F and similar in vaccinated and unvaccinated children.

Conclusions

About 4 of 10 healthy children had NP colonization with SP and around 40% serotypes were covered by PCV-13. Our preliminary data suggests that SP colonization rate and serotype isolates were similar in vaccinated and unvaccinated children

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PNEUMOCOCCAL CARRIAGE AND SEROTYPE DIVERSITY AMONG HEALTHY ADULTS IN BANGLADESH (ID 1095)

Session Name
Vaccines - Impact of Vaccine programs and Serotype Replacement

Abstract

Background

Streptococcus pneumoniae colonization in the nasopharynx (NP) of elderly reflects transmission in the community and disease risk. Few studies measured pneumococcal carriage among adults. However in developing countries, the data is scare. To address this, we conducted cross-sectional studies during October 2014-February 2015 and July-September 2015. As PCV-10 introduced in Bangladesh on March 2015, both periods are considered baseline data.

Methods

In Mirzapur, a rural area in Bangladesh, 1408 NP swabs were collected from healthy adults (age, 45-95 years) identified during demographic surveillance system household visits. Swabs were cultured for pneumococci and serotyped using quellung reaction.

Results

Pneumococcal colonization rates during the two time periods were 10% (80/762) and 9% (61/646), respectively. Fifty-one different serotypes were detected. Only 13% (19/141) of isolates were PCV-10 serotypes, 22% (31/141) were PCV-13, 33% (46/141) were PCV-20, and 34% (48/141) were 23-valent polysaccharide vaccine serotypes. The most predominant serotypes were 13 (8%), 34 (8%), 3 (6%), 39 (6%), 35B (4%), 18C (4%) and 19F (4%).

Conclusions

Our findings indicate that a diverse set of serotypes cause pneumococcal carriage among older adults in Bangladesh, with 4 of the 7 most predominant not found in existing or forthcoming conjugate vaccine formulations.

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PREVALENCE AND RISK FACTORS FOR PNEUMOCOCCAL CARRIAGE IN SOUTH ASIAN INFANTS: RESULTS OF OBSERVATIONAL COHORT STUDIES IN VACCINATED AND UNVACCINATED POPULATIONS (ID 1088)